Medical Microbiology Research Center, Qazvin University of Medical Sciences, Bahonar Blvd, P.O. Box: 34185-754, Qazvin, Iran.
Werner Siemens Chair of Synthetic Biotechnology, Department of Chemistry, Technical University of Munich, Munich, Germany.
Probiotics Antimicrob Proteins. 2024 Feb;16(1):224-232. doi: 10.1007/s12602-022-10030-w. Epub 2022 Dec 22.
Saccharomyces cerevisiae var. boulardii has been used as a probiotic yeast in the medical and food industries. Colon cancers have been known as the third most common cancer type worldwide. Nowadays, cell-free extract and metabolites of probiotics have been employed for the treatment or prevention of different cancer diseases. This study investigates the anticancer properties of S. boulardii metabolites against human colon carcinoma. We evaluated cytotoxicity, apoptosis induction, and suppression of survivin, IL-8, and NFƙB gene expression effects of SBM against caco-2 cells after 24 and 48 h. IC concentrations of SBM were measured at 815 and 1411 µg/mL for 24 and 48 h treatments, respectively. The total proportion of apoptotic caco-2 cells treated with SBM after 24 and 48 h were calculated at 62.23 and 88.7%, respectively. Also, relative expression of survivin, IL-8, and NFƙB genes were significantly suppressed in caco-2 cells treated with SBM after 24 and 48 h. In conclusion, we found that SBM induced apoptosis, inhibited the growth rate, and suppressed the expression of the survivin, IL-8, and NFƙB genes in human colorectal cancer cells and it can be considered as a perspective supplement or drug for the treatment or prevention of colon cancer in humans.
酿酒酵母变种巴氏酵母已被广泛应用于医学和食品工业中的益生菌酵母。结肠癌是全球第三大常见的癌症类型。如今，益生菌的无细胞提取物和代谢产物已被用于治疗或预防不同的癌症疾病。本研究旨在探讨巴氏酵母代谢物对人结肠癌细胞的抗癌特性。我们评估了 SBM 对 Caco-2 细胞的细胞毒性、凋亡诱导以及 Survivin、IL-8 和 NFƙB 基因表达抑制作用，分别在 24 和 48 小时后进行检测。SBM 的 IC 浓度在 24 和 48 小时的处理中分别为 815 和 1411µg/mL。用 SBM 处理 24 和 48 小时后的总凋亡 Caco-2 细胞比例分别为 62.23%和 88.7%。此外，SBM 处理后的 Survivin、IL-8 和 NFƙB 基因的相对表达水平也显著降低。综上所述，我们发现 SBM 能诱导人结直肠癌细胞凋亡、抑制其生长速度、并抑制 Survivin、IL-8 和 NFƙB 基因的表达，因此可被视为治疗或预防人类结肠癌的一种有前景的补充剂或药物。
