College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
Toxins (Basel). 2022 Dec 17;14(12):883. doi: 10.3390/toxins14120883.
Aflatoxin is a carcinogenic secondary metabolite that poses a serious threat to human and animal health. Some CH transcription factors are associated with fungal growth and secondary metabolic regulation. In this study, we characterized the role of , a putative CH transcription factor based on genome annotation, in the growth and aflatoxin biosynthesis of and explored its possible mechanisms of action. Surprisingly, the protein was found to be located in the cytoplasm, and gene deletion in resulted in defective growth and conidia formation, as well as increased sensitivity to the fluorescent brightener Calcofluor white, Congo red, NaCl, and sorbitol stress. Notably, the biosynthesis of aflatoxin B was completely inhibited in the Δ deletion strain, and its ability to infect peanut and corn seeds was also reduced. RNA sequencing showed that differentially expressed genes in the Δ strain compared with the control and complementation strains were mainly associated with growth, aflatoxin biosynthesis, and oxidative stress. Thus, likely contributes to growth, cell development, and aflatoxin synthesis in . These findings lay the foundation for a deeper understanding of the roles of CH transcription factors in and provide a potential biocontrol target for preventing aflatoxin contamination.
黄曲霉毒素是一种致癌的次级代谢物，对人类和动物健康构成严重威胁。一些 CH 转录因子与真菌生长和次级代谢调控有关。在这项研究中，我们根据基因组注释，对基于假定的 CH 转录因子 进行了特征描述，研究了其在 和生长和黄曲霉毒素生物合成中的作用，并探讨了其可能的作用机制。令人惊讶的是，该蛋白被发现位于细胞质中，而 在 中的缺失导致生长和分生孢子形成缺陷，以及对荧光增白剂 Calcofluor white、刚果红、NaCl 和山梨糖醇的敏感性增加。值得注意的是，Δ 缺失菌株中黄曲霉毒素 B 的生物合成完全被抑制，其侵染花生和玉米种子的能力也降低。RNA 测序显示，与对照和互补菌株相比，Δ 菌株中差异表达的基因主要与生长、黄曲霉毒素生物合成和氧化应激有关。因此， 在 中可能有助于生长、细胞发育和黄曲霉毒素的合成。这些发现为深入了解 CH 转录因子在 和生长中的作用奠定了基础，并为防止黄曲霉毒素污染提供了一个潜在的生物防治靶标。
