Department of Materials Engineering, School of Chemical and Materials Engineering (SCME), National University of Sciences and Technology (NUST), Islamabad 44000, Pakistan.
Department of Chemical and Biomolecular Engineering, National University of Singapore, Engineering Drive 4, Singapore 117576, Singapore.
Biosensors (Basel). 2022 Nov 23;12(12):1067. doi: 10.3390/bios12121067.
In the present study, a functional template made up of in situ synthesised gold nanoparticles (AuNPs) is prepared on polydimethylsiloxane (PDMS) for patterning of target protein onto the desired solid substrates. Unlike previous studies in which bioreceptor probes are randomly attached to the PDMS stamp through electrostatic interactions, herein, we propose an AuNPs-PDMS stamp, which provides a surface for the attachment of thiol-modified biorecognition probes to link to the stamp surface through a dative bond with a single anchoring point based on thiol chemistry. By using this platform, we have developed the ability for microcontact printing (µCP) to selectively capture and transfer target protein onto solid surfaces for detection purposes. After µCP, we also investigated whether liquid crystals (LCs) could be used as a label-free approach for identifying transfer protein. Our reported approach provides promise for biosensing of various analytes.
在本研究中，在聚二甲基硅氧烷（PDMS）上制备了由原位合成的金纳米粒子（AuNPs）组成的功能模板，以便将目标蛋白图案化到所需的固体基底上。与之前通过静电相互作用将生物受体探针随机附着到 PDMS 印章的研究不同，在此，我们提出了一种 AuNPs-PDMS 印章，它提供了一个表面，用于通过基于硫醇化学的配位键将巯基修饰的生物识别探针附着到印章表面，只有一个锚固点。通过使用这个平台，我们已经开发了微接触印刷（µCP）的能力，以便选择性地捕获和转移目标蛋白到固体表面上进行检测。µCP 之后，我们还研究了液晶（LCs）是否可以用作识别转移蛋白的无标记方法。我们报道的方法为各种分析物的生物传感提供了前景。
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