State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Mikrochim Acta. 2019 Jun 10;186(7):401. doi: 10.1007/s00604-019-3499-1.
A method is described for single-step detection of V. parahaemolyticus in seafood via aptamer-based SERS. A gold-coated polydimethylsiloxane (PDMS) film was used for the enhancement of Raman scattering. The Raman reporter 4-mercaptobenzoic acid was linked to aptamer modified gold nanoparticles (AuNPs) served as a signalling probe. The negatively charged signalling probe was assembled onto the cysteamine-modified Au-PDMS film through electrostatic adsorption. On addition of V. parahaemolyticus, it will be bound by the aptamer as a biorecognition element, and this leads to the dissociation of the signalling probe from the Au-PDMS film. Hence, the Raman signal (at 1592 cm) decreases. The assay has a wide linear response that covers the 1.2 × 10 to 1.2 × 10 cfu·mL V. parahaemolyticus concentration range. The detection limit is 12 cfu·mL. The method was successfully applied to the determination of V. parahaemolyticus in oyster and salmon samples. Graphical abstract Schematic presentation of a surface-enhanced Raman spectroscopic method for single step detection of Vibrio parahaemolyticus using gold coated polydimethylsiloxane as the active substrate and aptamer modified gold nanoparticles. This solid substrate simplified the analysis procedures and enhanced the sensitivity.
描述了一种通过适配体增强表面增强拉曼散射（SERS）一步法检测海产品中副溶血性弧菌的方法。采用金涂覆聚二甲基硅氧烷（PDMS）薄膜增强拉曼散射。将拉曼报告分子 4-巯基苯甲酸连接到作为信号探针的适配体修饰金纳米粒子（AuNPs）上。带负电荷的信号探针通过静电吸附组装到半胱胺修饰的 Au-PDMS 薄膜上。加入副溶血性弧菌后，它将作为生物识别元件与适配体结合，这导致信号探针从 Au-PDMS 薄膜上解离。因此，拉曼信号（在 1592 cm 处）降低。该测定法具有广泛的线性响应范围，涵盖了 1.2×10 至 1.2×10 cfu·mL 副溶血性弧菌浓度范围。检测限为 12 cfu·mL。该方法成功应用于牡蛎和三文鱼样品中副溶血性弧菌的测定。
