Jacq. is an aromatic shrub that has been utilized in traditional medicine in the Bahamas, Jamaica, and Cuba. Recent studies have revealed the antiprotozoal potential of its leaves. The present work is aimed to identify the volatile constituents of essential oil from the stems of (CLS-EO) and evaluate its in vitro antileishmanial activity. In addition, an in silico study of the molecular interactions was performed using molecular docking. A gas chromatographic-mass spectrometric analysis of CLS-EO identified 1,8-cineole (27.8%), -pinene (11.1%), -sabinene (8.1%), -cymene (5.7%), -terpineol (4.4%), --eudesmol (4.2%), linalool (3.9%), and terpinen-4-ol (2.6%) as major constituents. The evaluation of antileishmanial activity showed that CLS-EO has good activity on both parasite forms (IC = 21.4 ± 0.1 μg/mL; IC = 18.9 ± 0.3 μg/mL), with a CC of 49.0 ± 5.0 μg/mL on peritoneal macrophages from BALB/c mice (selectivity index = 2 and 3 using the promastigote and amastigote results). Molecular docking showed good binding of --eudesmol with different target enzymes of . This study is the first report of the chemical composition and anti- evaluation of CLS-EO. These findings provide support for further studies of the antileishmanial effect of this product.