[具体物质]对[具体物质]和[具体物质]的抗毒力作用

The Anti-Virulence Effect of against and .

作者信息

Motta Elizangela Pestana, Farias Josivan Regis, Costa Arthur André Castro da, Silva Anderson França da, Oliveira Lopes Alberto Jorge, Cartágenes Maria do Socorro Sousa, Nicolete Roberto, Abreu Afonso Gomes, Fernandes Elizabeth Soares, Nascimento Flavia Raquel Fernandes, Rocha Cláudia Quintino da, Monteiro Cristina Andrade, Guerra Rosane Nassar Meireles

机构信息

Laboratório de Imunofisiologia, Departamento de Patologia, Universidade Federal do Maranhão, Avenida dos Portugueses, 1966, Ensino Integrado, Bloco 1, São Luís 65080-805, MA, Brazil.

Programa de Pós-Graduação em Ciências da Saúde, Centro de Ciências Biológicas e da Saúde, Universidade Federal do Maranhão, Avenida dos Portugueses, 1966, São Luís 65080-805, MA, Brazil.

出版信息

Antibiotics (Basel). 2022 Dec 16;11(12):1834. doi: 10.3390/antibiotics11121834.

In folk medicine, is used to treat skin diseases and mycoses in the Amazon region. We evaluated the anti- activity of the hydroalcoholic extract from the leaves of (EHVG). HPLC-PDA and FIA-ESI-IT-MS were used to chemically characterize EHVG. The anti- activity was determined in vitro by the minimum inhibitory concentrations (MIC) against (ATCC-2001); (ATCC-90028, ATCC-14053, and ATCC-SC5314), and clinical isolates. EHVG effects on adhesion, growth, and biofilm formation were also determined. Molecular docking was used to predict targets for EHVG compounds. The main compounds identified included anthraquinone, vismione D, kaempferol, quercetin, and vitexin. EHVG was fungicidal against all tested strains. ATCC 14053 and ATCC 2001 were the most sensitive strains, as the extract inhibited their virulence factors. In silico analysis indicated that vismione D presented the best antifungal activity, since it was the most effective in inhibiting CaCYP51, and may act as anti-inflammatory and antioxidant agent, according to the online PASS prediction. Overall, the data demonstrate that EHVG has an anti- effect by inhibiting virulence factors of the fungi. This activity may be related to its vismione D content, indicating this compound may represent a new perspective for treating diseases caused by sp.

在民间医学中，[植物名称未给出]被用于治疗亚马逊地区的皮肤病和真菌病。我们评估了[植物名称未给出]叶的水醇提取物（EHVG）的抗[真菌名称未给出]活性。采用高效液相色谱-光电二极管阵列检测法（HPLC-PDA）和流动注射-电喷雾离子阱质谱法（FIA-ESI-IT-MS）对EHVG进行化学表征。通过针对[真菌名称未给出]（ATCC-2001）、[白色念珠菌相关菌株，名称未完整给出]（ATCC-90028、ATCC-14053和ATCC-SC5314）以及[真菌名称未给出]临床分离株的最低抑菌浓度（MIC）来体外测定抗[真菌名称未给出]活性。还测定了EHVG对黏附、生长和生物膜形成的影响。采用分子对接来预测EHVG化合物的作用靶点。鉴定出的主要化合物包括蒽醌、紫铆素D、山奈酚、槲皮素和牡荆素。EHVG对所有测试菌株均具有杀菌作用。[真菌名称未给出]ATCC 14053和[真菌名称未给出]ATCC 2001是最敏感的菌株，因为该提取物抑制了它们的毒力因子。计算机模拟分析表明紫铆素D具有最佳的抗真菌活性，因为它在抑制CaCYP51方面最有效，并且根据在线PASS预测可能作为抗炎和抗氧化剂发挥作用。总体而言，数据表明EHVG通过抑制真菌的毒力因子具有抗[真菌名称未给出]作用。这种活性可能与其紫铆素D含量有关，表明该化合物可能为治疗由[真菌名称未给出]属引起的疾病提供新的视角。