Hannachi Essia, Khan Firdos Alam, Slimani Yassine, Rehman Suriya, Trabelsi Zayneb, Akhtar Sultan, Al-Suhaimi Ebtesam A
Department of Nuclear Medicine Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia.
Department of Stem Cell Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia.
Biology (Basel). 2022 Dec 16;11(12):1836. doi: 10.3390/biology11121836.
Zinc oxide nanoparticles (ZnO NPs) are a promising platform for their use in biomedical research, especially given their anticancer and antimicrobial activities. This work presents the synthesis of ZnO NPs doped with different amounts of rare-earth ions of ytterbium (Yb) and cerium (Ce) and the assessment of their anticancer and antimicrobial activities. The structural investigations indicated a hexagonal wurtzite structure for all prepared NPs. The particle size was reduced by raising the amount of Ce and Yb in ZnO. The anticancer capabilities of the samples were examined by the cell viability MTT assay. Post 48-h treatment showed a reduction in the cancer cell viability, which was x = 0.00 (68%), x = 0.01 (58.70%), x = 0.03 (80.94%) and x = 0.05 (64.91%), respectively. We found that samples doped with x = 0.01 and x = 0.05 of Yb and Ce showed a better inhibitory effect on HCT-116 cancer cells than unadded ZnO (x = 0.00). The IC for HCT-116 cells of Ce and Yb co-doped ZnO nanoparticles was calculated and the IC values were x = 0.01 (3.50 µg/mL), x = 0.05 (8.25 µg/mL), x = 0.00 (11.75 µg/mL), and x = 0.03 (21.50 µg/mL). The treatment-doped ZnO NPs caused apoptotic cell death in the HCT-116 cells. The nanoparticles showed inhibitory action on both and . It can be concluded that doping ZnO NPs with Yb and Ce improves their apoptotic effects on cancer and microbial cells.
氧化锌纳米颗粒（ZnO NPs）因其在生物医学研究中的应用前景而备受关注，特别是考虑到它们的抗癌和抗菌活性。这项工作展示了掺杂不同量镱（Yb）和铈（Ce）等稀土离子的ZnO NPs的合成及其抗癌和抗菌活性评估。结构研究表明，所有制备的纳米颗粒均为六方纤锌矿结构。通过增加ZnO中Ce和Yb的含量，颗粒尺寸减小。通过细胞活力MTT测定法检测样品的抗癌能力。48小时处理后，癌细胞活力降低，分别为x = 0.00（68%），x = 0.01（58.70%），x = 0.03（80.94%）和x = 0.05（64.91%）。我们发现，掺杂x = 0.01和x = 0.05的Yb和Ce的样品对HCT-116癌细胞的抑制作用比未添加ZnO（x = 0.00）更好。计算了Ce和Yb共掺杂ZnO纳米颗粒对HCT-116细胞的IC值，IC值分别为x = 0.01（3.50 µg/mL），x = 0.05（8.25 µg/mL），x = 0.00（11.75 µg/mL）和x = 0.03（21.50 µg/mL）。掺杂ZnO NPs的处理导致HCT-116细胞凋亡性死亡。纳米颗粒对[具体对象1]和[具体对象2]均显示出抑制作用。可以得出结论，用Yb和Ce掺杂ZnO NPs可改善其对癌细胞和微生物细胞的凋亡作用。
