V.M. Gorbatov Federal Research Center for Food Systems, Russian Academy of Sciences, 109316 Moscow, Russia.
Institute of Biomedical Chemistry, 119121 Mosow, Russia.
Genes (Basel). 2022 Nov 24;13(12):2204. doi: 10.3390/genes13122204.
(1) Background: Stroke is the leading cause of serious long-term disability. Walking dysfunction and paresis of the upper extremities occurs in more than 80% of people who have had a stroke. (2) Methods: We studied post-genomic markers in biosamples of muscle and brain tissue from animals that underwent intracerebral hematoma and recovered after 42 days. Our purpose was to understand the biological mechanisms associated with recovery from hemorrhagic stroke. We analyzed the peptides formed after trypsinolysis of samples by HPLC-MS, and the results were processed by bioinformatics methods, including the establishment of biochemical relationships (gene to gene) using topological omics databases such as Reactome and KEGG. (3) Results: In the pig brain, unique compounds were identified which are expressed during the recovery period after traumatic injury. These are molecular factors of activated microglia, and they contribute to the functional recovery of neurons and reduce instances of hematoma, edema, and oxidative stress. Complexes of the main binding factors of the neurotrophins involved in the differentiation and survival of nerve cells were found in muscles. (4) Conclusions: A network of gene interactions has been constructed for proteins involved in the regulation of synaptic transmission, in particular presynaptic vesicular and endocytic processes. The presence of transmitters and transporters associated with stimulation of NMDA receptors at neuromuscular junctions shows the relationship between upper motor neurons and neuromuscular junctions.
(1) 背景：中风是导致严重长期残疾的主要原因。超过 80%的中风患者会出现行走功能障碍和上肢瘫痪。(2) 方法：我们研究了经过颅内血肿并在 42 天后恢复的动物的肌肉和脑组织生物样本中的后基因组标记物。我们的目的是了解与脑出血后恢复相关的生物学机制。我们通过 HPLC-MS 分析了样品经胰蛋白酶消化后形成的肽，并通过生物信息学方法处理结果，包括使用 Reactome 和 KEGG 等拓扑组学数据库建立生化关系（基因到基因）。(3) 结果：在猪脑中，鉴定出了在创伤后恢复期间表达的独特化合物。这些是激活的小胶质细胞的分子因素，它们有助于神经元的功能恢复，并减少血肿、水肿和氧化应激的发生。在肌肉中发现了涉及神经元分化和存活的神经营养因子的主要结合因子的复合物。(4) 结论：构建了一个涉及调节突触传递的蛋白质的基因相互作用网络，特别是涉及突触小泡和内吞作用过程的蛋白质。与 NMDA 受体刺激相关的递质和转运体在神经肌肉接头的存在表明了上运动神经元和神经肌肉接头之间的关系。
