Horticulture Department, College of Agricultural Engineering Sciences, University of Sulaimani, Sulaimani 46001, Iraq.
USDA-ARS, Plant Germplasm Introduction and Testing Research Unit, 201 Clark Hall, Washington State University, Pullman, WA 99164, USA.
Genes (Basel). 2022 Dec 6;13(12):2297. doi: 10.3390/genes13122297.
White mold can result in snap bean yield losses of 90 to 100% when field conditions favor the pathogen. A genome-wide association study (GWAS) was conducted to detect loci significantly associated with white mold resistance in a panel of snap bean ( L.) cultivars. Two populations of snap bean were used in this study. The first population was the BeanCAP (Coordinated Agriculture Project) Snap Bean Diversity Panel (SBDP) ( = 136), and the second population was the Snap Bean Association Panel (SnAP) ( = 378). SBDP was evaluated for white mold reaction in the field in 2012 and 2013, and SnAP was screened in a greenhouse only using the seedling straw test in 2016. Two reference genomes representing the Andean and Middle American centers of domestication were utilized to align the genotyping-by-sequencing (GBS) data. A GWAS was performed using FarmCPU with one principal component after comparing five models. Thirty-four single-nucleotide polymorphisms (SNPs) significantly associated with white mold resistance were detected. Eleven significant SNPs were identified by the seedling straw test, and 23 significant SNPs were identified by field data. Fifteen SNPs were identified within a 100 kb window containing pentatricopeptide repeat (PPR)-encoding genes, and eleven were close to leucine-rich repeat (LRR)-encoding genes, suggesting that these two classes are of outsized importance for snap bean resistance to white mold.
白霉病在有利于病原菌的田间条件下可导致菜豆产量损失 90%至 100%。本研究进行了全基因组关联分析（GWAS），以检测菜豆（L.）品种群体中与白霉病抗性显著相关的基因座。本研究使用了两个菜豆群体。第一个群体是 BeanCAP（协调农业项目）菜豆多样性面板（SBDP）（=136），第二个群体是菜豆关联面板（SnAP）（=378）。SBDP 在 2012 年和 2013 年的田间评估了对白霉病的反应，而 SnAP 仅在 2016 年的温室中使用幼苗秸秆试验进行了筛选。利用代表安第斯山脉和中美洲驯化中心的两个参考基因组，对基于测序的基因型（GBS）数据进行了比对。使用 FarmCPU 进行了 GWAS，在比较了五个模型后，使用一个主成分进行分析。共检测到 34 个与白霉病抗性显著相关的单核苷酸多态性（SNP）。通过幼苗秸秆试验鉴定到 11 个显著 SNP，通过田间数据鉴定到 23 个显著 SNP。在包含五肽重复（PPR）编码基因的 100 kb 窗口内鉴定到 15 个 SNP，11 个 SNP 接近富含亮氨酸重复（LRR）编码基因，表明这两个基因座对菜豆对白霉病的抗性具有重要作用。
