School of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Department of Biochemistry & Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA.
Genes (Basel). 2022 Dec 16;13(12):2376. doi: 10.3390/genes13122376.
Kinesin, as a member of the molecular motor protein superfamily, plays an essential function in various plants' developmental processes. Especially at the early stages of plant growth, including influences on plants' growth rate, yield, and quality. In this study, we did a genome-wide identification and expression profile analysis of the kinesin family in barley. Forty-two HvKINs were identified and screened from the barley genome, and a generated phylogenetic tree was used to compare the evolutionary relationships between Rice and Arabidopsis. The protein structure prediction, physicochemical properties, and bioinformatics of the HvKINs were also dissected. Our results reveal the important regulatory roles of genes in barley growth. We found many elements related to and in homeopathic elements of the gene and verified them by QRT-PCR, indicating their potential role in the barley kinesin family. The current study revealed the biological functions of barley kinesin genes in barley and will aid in further investigating the kinesin in other plant species.
驱动蛋白作为分子马达蛋白超家族的一员,在各种植物的发育过程中发挥着重要的功能。特别是在植物生长的早期阶段,包括对植物生长速度、产量和质量的影响。在这项研究中,我们对大麦中的驱动蛋白家族进行了全基因组鉴定和表达谱分析。从大麦基因组中鉴定和筛选出了 42 个 HvKINs,并构建了一个进化树来比较水稻和拟南芥之间的进化关系。还对 HvKINs 的蛋白结构预测、理化性质和生物信息学进行了剖析。我们的研究结果揭示了这些基因在大麦生长中的重要调控作用。我们发现了许多与基因的同源和非同源元件相关的元素,并通过 QRT-PCR 对其进行了验证,表明它们在大麦驱动蛋白家族中可能具有潜在的作用。本研究揭示了大麦驱动蛋白基因在大麦中的生物学功能,并将有助于进一步研究其他植物物种中的驱动蛋白。