Genome-wide identification and expression analysis of the gene family in pea.

Peas ( L.) serve as a vital model for plant development and stress research. The () gene family, encoding essential motor proteins, remains understudied in peas. Our research conducted a comprehensive genomic analysis of the genes in peas, identifying 105 genes categorized into seven subfamilies based on evolutionary relationships, gene structures, conserved motifs, and interaction networks. A comparative analysis with and gene families showed a non-uniform distribution of genes across subfamilies. Homology analysis revealed that the family has undergone segmental duplication and is under negative selection pressures, with conserved genes on chromosomes Ps5, Ps6, and Ps7 playing a significant role in pea evolution. Transcriptomics revealed 38 genes with distinct tissue-specific expression, with , , , and showing significant levels in roots, lateral roots, stems, petals, and seeds, respectively. Differential expression under drought and saline stress was observed, with , , upregulated under drought, and and under saline stress. These genes are potential candidates for improving plant stress tolerance. This study offers insights into the pea gene family, highlighting their potential in enhancing plant stress tolerance and setting a stage for future research.