Chan Pei-Teng, Matanjun Patricia, Budiman Cahyo, Shapawi Rossita, Lee Jau-Shya
Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia.
Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia.
Foods. 2022 Dec 9;11(24):3991. doi: 10.3390/foods11243991.
The heads and bones of hybrid groupers are potential precursors for angiotensin-converting enzyme (ACE)-inhibitory and antioxidant peptides. The aim of this study was to isolate the dual-action peptides from the Alcalase-treated head and bone hydrolysate of hybrid groupers followed by identification of the novel peptides. The stability of these peptides against stimulated in vitro gastrointestinal digestion (SGID) was also determined. Fraction HB-IV (less than 1 kDa) obtained from ultrafiltration showed the strongest ACE-inhibition ability (IC50: 0.28 mg/mL), which was comparable to the potency of the commercial supplement, PeptACE (IC50: 0.22 mg/mL). This fraction also demonstrated the highest hydroxyl radical scavenging and metal-chelating activities. However, further fractionation of HB-IV by a series of chromatography resulted in peptide fractions of reduced ACE-inhibitory and antioxidant activities. The hydroxyl radical scavenging and reduction potential of HB-IV were enhanced, whereas ACE-inhibitory and metal-chelating activities were reduced following SGID. A total of 145 peptide sequences were identified from HB-IV, of which 137 peptides were novel to the BIOPEP database. The results suggested that the bioactive peptides isolated from the heads and bones of hybrid groupers could be used as functional foods/ingredients with potential ACE-inhibitory and antioxidant effects.
杂交石斑鱼的鱼头和鱼骨是血管紧张素转换酶（ACE）抑制肽和抗氧化肽的潜在来源。本研究旨在从经碱性蛋白酶处理的杂交石斑鱼鱼头和鱼骨水解物中分离出具有双重作用的肽，并鉴定新的肽。还测定了这些肽在体外模拟胃肠道消化（SGID）条件下的稳定性。超滤得到的HB-IV级分（小于1 kDa）表现出最强的ACE抑制能力（IC50：0.28 mg/mL），与商业补充剂PeptACE的效力相当（IC50：0.22 mg/mL）。该级分还表现出最高的羟基自由基清除能力和金属螯合活性。然而，通过一系列色谱法对HB-IV进行进一步分级分离后，肽级分的ACE抑制和抗氧化活性降低。在SGID后，HB-IV的羟基自由基清除能力和还原电位增强，而ACE抑制和金属螯合活性降低。从HB-IV中总共鉴定出145个肽序列，其中137个肽在BIOPEP数据库中是新的。结果表明，从杂交石斑鱼的鱼头和鱼骨中分离出的生物活性肽可作为具有潜在ACE抑制和抗氧化作用的功能性食品/成分。
