National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.
Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.
Mar Drugs. 2019 May 3;17(5):263. doi: 10.3390/md17050263.
In the work, water-soluble proteins of red stingray () cartilages were extracted by guanidine hydrochloride and hydrolyzed using trypsin. Subsequently, four antioxidant peptides (RSHP-A, RSHP-B, RSHP-C, and RSHP-D) were isolated from the water-soluble protein hydrolysate while using ultrafiltration and chromatographic techniques, and the amino acid sequences of RSHP-A, RSHP-B, RSHP-C, and RSHP-D were identified as Val-Pro-Arg (VPR), Ile-Glu-Pro-His (IEPH), Leu-Glu-Glu--Glu-Glu (LEEEE), and Ile-Glu-Glu-Glu-Gln (IEEEQ), with molecular weights of 370.46 Da, 494.55 Da, 647.64 Da, and 646.66 Da, respectively. VPR, IEPH, LEEEE, and IEEEQ exhibited good scavenging activities on the DPPH radical (EC values of 4.61, 1.90, 3.69, and 4.01 mg/mL, respectively), hydroxyl radical (EC values of 0.77, 0.46, 0.70, and 1.30 mg/mL, respectively), superoxide anion radical (EC values of 0.08, 0.17, 0.15, and 0.16 mg/mL, respectively), and ABTS cation radical (EC values of 0.15, 0.11, 0.19, and 0.18 mg/mL, respectively). Among the four isolated antioxidant peptides, IEPH showed the strongest reducing power and lipid peroxidation inhibition activity, but LEEEE showed the highest Fe-chelating ability. The present results suggested that VPR, IEPH, LEEEE, and IEEEQ might have the possibility of being an antioxidant additive that is used in functional food and pharmaceuticals.
在这项工作中，采用盐酸胍提取红鳐软骨中的水溶性蛋白质，并利用胰蛋白酶进行水解。随后，通过超滤和色谱技术从水溶性蛋白水解物中分离出四种抗氧化肽（RSHP-A、RSHP-B、RSHP-C 和 RSHP-D），并鉴定出 RSHP-A、RSHP-B、RSHP-C 和 RSHP-D 的氨基酸序列分别为缬氨酸-脯氨酸-精氨酸（VPR）、异亮氨酸-谷氨酸-脯氨酸-组氨酸（IEPH）、亮氨酸-谷氨酸-谷氨酸-谷氨酸-谷氨酸（LEEEE）和异亮氨酸-谷氨酸-谷氨酸-谷氨酸-谷氨酰胺（IEEEQ），它们的分子量分别为 370.46 Da、494.55 Da、647.64 Da 和 646.66 Da。VPR、IEPH、LEEEE 和 IEEEQ 对 DPPH 自由基（EC 值分别为 4.61、1.90、3.69 和 4.01 mg/mL）、羟基自由基（EC 值分别为 0.77、0.46、0.70 和 1.30 mg/mL）、超氧阴离子自由基（EC 值分别为 0.08、0.17、0.15 和 0.16 mg/mL）和 ABTS 阳离子自由基（EC 值分别为 0.15、0.11、0.19 和 0.18 mg/mL）均具有良好的清除活性。在分离得到的四种抗氧化肽中，IEPH 表现出最强的还原能力和脂质过氧化抑制活性，但 LEEEE 表现出最高的铁螯合能力。这些结果表明，VPR、IEPH、LEEEE 和 IEEEQ 可能具有作为抗氧化剂添加剂应用于功能性食品和药物的潜力。
