Tondossama Naminata, Coulibaly Zanakoungo I, Traoré Issouf, Ako Bérenger A, Zoh Danielle D, Virgillito Chiara, Guindo-Coulibaly Négnorogo, Serini Paola, Assouho Fabrice K, Dia Ibrahima, Touré Andre O, Adja Maurice A, Caputo Beniamino, Della Torre Alessandra, Pichler Verena
Laboratoire de Biologie et Santé, UFR Biosciences, Université Félix Houphouet Boigny Cocody, Abidjan 01 BP V34, Côte d'Ivoire.
Entomology and Herpetology Unit, Institut Pasteur de Côte d'Ivoire, Abidjan 01 PB 490, Côte d'Ivoire.
Insects. 2022 Nov 26;13(12):1090. doi: 10.3390/insects13121090.
and -the two most recently radiated species of the complex and the major Afrotropical malaria vector species-are identified by markers in the X-centromeric IGS rDNA region. Putative IGS-hybrids are rarely found in the field, except in restricted areas where genomic studies have led to the hypothesis that the observed IGS-patterns are due to cryptic taxa rather than to hybridization between the two species. We investigated the genome-wide levels of admixture in two villages in Côte d'Ivoire where high levels of IGS-hybrids have been detected, confirming unparalleled high frequencies in the coastal village. Genotyping of 24 Ancestry Informative Markers (AIMs) along the three chromosomes produced discordant results between the IGS-marker and the multilocus genotype obtained for AIMs across the whole genome (29%) as well as AIMs on chromosome-X (considered to be fundamental for species reproductive isolation) only (21%). Results highlight a complicated pattern of admixture that deserves deeper genomic analyses to understand better possible underlying causes (from extensive processes of hybridization to the existence of different cryptic taxa), and stress the need of developing advanced diagnostics for and putative new taxa, instrumental for assessing taxon-specific epidemiological characters.
复合体中最近辐射分化的两个物种以及主要的非洲热带疟疾媒介物种，可通过X着丝粒IGS rDNA区域的标记来识别。除了在一些特定区域，在野外很少发现假定的IGS杂种，在这些特定区域，基因组研究提出了这样的假说，即观察到的IGS模式是由于隐存分类群而非两个物种之间的杂交所致。我们在科特迪瓦的两个村庄调查了全基因组的混合水平，在这两个村庄检测到了高水平的IGS杂种，证实了沿海村庄中出现了前所未有的高频率。沿着三条染色体对24个祖先信息标记（AIMs）进行基因分型，结果显示IGS标记与全基因组AIMs（29%）以及仅在X染色体上的AIMs（被认为对物种生殖隔离至关重要）（21%）所获得的多位点基因型之间存在不一致。结果突出了一种复杂的混合模式，这值得进行更深入的基因组分析，以更好地理解可能的潜在原因（从广泛的杂交过程到不同隐存分类群的存在），并强调需要为假定的新分类群开发先进的诊断方法，这对于评估分类群特异性的流行病学特征至关重要。
