Department of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto 14049-900, SP, Brazil.
Department of Physiological Science, Center of Biological Sciences, Federal University of Santa Catarina, Florianópolis 88040-900, SC, Brazil.
Int J Mol Sci. 2022 Dec 10;23(24):15672. doi: 10.3390/ijms232415672.
Aging is a complex biological process, resulting in gradual and progressive decline in structure and function in many organ systems. Our objective is to determine if structural changes produced by aging vary with sex in a stressful situation such as dehydration. The expression of mRNA in the renal cortex, α-smooth muscle actin (α-SMA), and fibronectin was evaluated in male and female rats, aged 3 and 18 months, submitted and not submitted to water deprivation (WD) for 48 h, respectively. When comparing ages, 18-month-old males showed a lower expression of mRNA than 3-month-old males, and control and WD 18-month-old male and female rats exhibited a higher expression of α-SMA than the respective 3-month-old rats. Fibronectin was higher in both control and WD 18-month-old males than the respective 3-month-old males. In females, only the control 18-month-old rats showed higher fibronectin than the control 3-month-old rats. When we compared sex, control and WD 3-month-old female rats had a lower expression of mRNA than the respective males. The WD 18-month-old male rats presented a higher expression of fibronectin and α-SMA than the WD 18-month-old female rats. When we compared hydric conditions, the WD 18-month-old males displayed a lower relative expression of mRNA and higher α-SMA expression than the control 18-month-old males. Aging, sex, and dehydration lead to alterations in kidney structure.
衰老是一个复杂的生物学过程，导致许多器官系统的结构和功能逐渐和进行性下降。我们的目的是确定在脱水等应激情况下，衰老引起的结构变化是否因性别而异。评估了雄性和雌性大鼠肾皮质中的 mRNA 表达、α-平滑肌肌动蛋白 (α-SMA) 和纤维连接蛋白，这些大鼠分别为 3 个月和 18 个月龄，分别接受和不接受 48 小时的水剥夺 (WD)。当比较年龄时，18 个月龄雄性大鼠的 mRNA 表达低于 3 个月龄雄性大鼠，而对照和 WD 的 18 个月龄雄性和雌性大鼠的 α-SMA 表达均高于相应的 3 个月龄大鼠。纤维连接蛋白在对照和 WD 的 18 个月龄雄性大鼠中均高于相应的 3 个月龄雄性大鼠。在雌性大鼠中，只有对照的 18 个月龄大鼠的纤维连接蛋白高于相应的 3 个月龄大鼠。当比较性别时，对照和 WD 的 3 个月龄雌性大鼠的 mRNA 表达低于相应的雄性大鼠。WD 的 18 个月龄雄性大鼠的纤维连接蛋白和 α-SMA 表达均高于 WD 的 18 个月龄雌性大鼠。当比较水合条件时，WD 的 18 个月龄雄性大鼠的 mRNA 相对表达水平较低，α-SMA 表达水平较高，与对照的 18 个月龄雄性大鼠相比。衰老、性别和脱水导致肾脏结构发生变化。
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