Hospital of Ministry of Interior and Administration, 40-052 Katowice, Poland.
ICZ Healthcare Hospital in Zywiec, 34-300 Zywiec, Poland.
Int J Mol Sci. 2022 Dec 13;23(24):15817. doi: 10.3390/ijms232415817.
Reactive oxygen species are formed as by-products of normal cell metabolism. They are needed to maintain cell homeostasis and signaling, which is possible due to defense systems. Disruption of this balance leads to oxidative stress that can induce cancer. Redox regulation by miRNAs may be a potential therapeutic target. The aim of the study was to assess the activity of genes associated with oxidative stress in endometrial cancer and to determine their relationship with miRNAs. The study included 45 patients with endometrioid endometrial cancer and 45 without neoplastic changes. The expression profile of genes associated with oxidative stress was determined with mRNA microarrays, RT-qPCR and ELISA. The miRNA prediction was performed based on the miRNA microarray experiment and the mirDB tool. PRDX2 and AQP1 showed overexpression that was probably not related to miRNA activity. A high level of PKD2 may be the result of a decrease in the activity of miR-195-3p, miR-20a, miR-134. A SOD3 level reduction can be caused by miR-328, miR-363. In addition, miR-363 can also regulate KLF2 expression. In the course of endometrial cancer, the phenomenon of oxidative stress is observed, the regulation of which may be influenced by miRNAs.
活性氧是正常细胞代谢的副产物。它们是维持细胞内稳态和信号传递所必需的，这是由于防御系统的存在。这种平衡的破坏会导致氧化应激，从而诱发癌症。miRNA 的氧化还原调节可能是一种潜在的治疗靶点。本研究旨在评估与子宫内膜癌相关的氧化应激基因的活性，并确定它们与 miRNA 的关系。该研究纳入了 45 例子宫内膜样子宫内膜癌患者和 45 例无肿瘤性改变的患者。采用 mRNA 微阵列、RT-qPCR 和 ELISA 检测与氧化应激相关的基因表达谱。基于 miRNA 微阵列实验和 mirDB 工具进行 miRNA 预测。PRDX2 和 AQP1 的过表达可能与 miRNA 活性无关。PKD2 水平升高可能是 miR-195-3p、miR-20a、miR-134 活性降低的结果。SOD3 水平降低可能是由 miR-328、miR-363 引起的。此外，miR-363 还可以调节 KLF2 的表达。在子宫内膜癌的发生过程中观察到氧化应激现象，其调控可能受 miRNA 的影响。
