Wu Chieh-Shan, Lin Chuan-Chao, Chen Yu-Ying, Yang Deng-Ho
Department of Dermatology, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan.
Division of Dermatology, Pingtung Veterans General Hospital, Pingtung 900, Taiwan.
Life (Basel). 2022 Dec 15;12(12):2107. doi: 10.3390/life12122107.
Isorhamnetin (IRh), which has a wide range of pharmacological effects, is one of the most significant active components in the fruits of L. and the leaves of L. It protects the heart and brain, in addition to possessing anti-tumor, anti-inflammatory, antioxidant, organ protection, and anti-obesity properties. We sought to assess IRh's anti-psoriatic activity, explore its immunomodulatory properties in reducing the severity of psoriatic symptoms, and evaluate its potential immunotherapeutic effects. We used IRh to treat imiquimod (IMQ)-induced psoriasis in BALB/C mice and examined the underlying mechanisms. The outcomes demonstrated that IRh reduced epidermal hyperplasia, lowered PASI scores, and improved histopathological psoriasiform lesions in IMQ-induced mice. IRh attenuated the accumulation of malondialdehyde (MDA), and also reversed the reduction caused by IMQ of superoxide dismutase (SOD) and catalase (CAT) in skin tissues. Additionally, IRh effectively inhibited IMQ's ability to increase proinflammatory cytokines such as TNF-α, IL-6, IL-17A, and transcription factor NF-κB. Furthermore, IRh significantly reduced the percentage of Th1 and Th17 in the spleens of mice treated with IMQ and suppressed the maturation of splenic dendritic cells. Overall, our research suggests that IRh protects against oxidative stress and inflammation in the pathogenesis of psoriasis, with potential for the development of new and potent medication for the treatment of psoriasis.
异鼠李素（IRh）具有广泛的药理作用，是枸杞果实和枸杞叶中最重要的活性成分之一。它除了具有抗肿瘤、抗炎、抗氧化、器官保护和抗肥胖特性外，还能保护心脏和大脑。我们试图评估IRh的抗银屑病活性，探索其在减轻银屑病症状严重程度方面的免疫调节特性，并评估其潜在的免疫治疗效果。我们使用IRh治疗咪喹莫特（IMQ）诱导的BALB/C小鼠银屑病，并研究其潜在机制。结果表明，IRh可减轻IMQ诱导的小鼠表皮增生，降低银屑病面积和严重程度指数（PASI）评分，并改善组织病理学上的银屑病样病变。IRh减少了丙二醛（MDA）的积累，还逆转了IMQ导致的皮肤组织中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的减少。此外，IRh有效抑制了IMQ增加促炎细胞因子如肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-17A（IL-17A）和转录因子核因子-κB（NF-κB）的能力。此外，IRh显著降低了IMQ处理小鼠脾脏中Th1和Th17细胞的百分比，并抑制了脾脏树突状细胞的成熟。总体而言，我们的研究表明，IRh在银屑病发病机制中可抵御氧化应激和炎症，具有开发新型高效银屑病治疗药物的潜力。
