Yildirim Funda, Sudagidan Mert, Aydin Ali, Akyazi Ibrahim, Bayrakal Gulay Merve, Yavuz Orhan, Gurel Aydin
Department of Pathology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, Istanbul 34500, Turkey.
Scientific and Technology Application and Research Center, Mehmet Akif Ersoy University, Burdur 15030, Turkey.
Life (Basel). 2022 Dec 16;12(12):2126. doi: 10.3390/life12122126.
Toxin-producing strains posing a potential risk for public health have long been a topic of scientific research. Effects of Panton-Valentine leukocidin (PVL) on tissue destruction mechanisms and activities of inflammatory cells were presented in animal models of pneumonia and skin infections induced by PVL-producing strains. This study aimed to demonstrate the in vivo pathogenicity of PVL-producing strains isolated from some foodstuffs, which can be a potential risk to public health. PVL-positive methicillin-susceptible (MSSA) strains M1 and YF1B-b isolated from different foodstuffs and a PVL-positive MSSA strain HT480 (positive control) were administered to New Zealand rabbits. Blood samples were harvested three and six hours after the intratracheal inoculation. Lung tissue samples were collected for gross and microscopic exams and immunohistochemical (IHC) demonstration of IL-6, IL8, IL-10, and TNF-α expressions. Serum cytokine levels were also measured by ELISA. The strains isolated from lung tissue samples were confirmed by pulsed-field gel electrophoresis. The development of acute necrotising pneumonia and a significant elevation in IL-6, IL-8, IL-10, and TNF-α expressions demonstrated the significance of foodborne PVL-positive MSSA strains in public health for the first time.
产生毒素的菌株对公众健康构成潜在风险，长期以来一直是科学研究的课题。在由产Panton-Valentine杀白细胞素（PVL）的菌株引起的肺炎和皮肤感染动物模型中，展示了PVL对组织破坏机制和炎性细胞活性的影响。本研究旨在证明从某些食品中分离出的产PVL菌株的体内致病性，这些菌株可能对公众健康构成潜在风险。将从不同食品中分离出的PVL阳性甲氧西林敏感（MSSA）菌株M1和YF1B-b以及PVL阳性MSSA菌株HT480（阳性对照）接种到新西兰兔体内。气管内接种后3小时和6小时采集血样。收集肺组织样本进行大体和显微镜检查以及IL-6、IL-8、IL-10和TNF-α表达的免疫组织化学（IHC）显示。血清细胞因子水平也通过ELISA进行测量。通过脉冲场凝胶电泳确认从肺组织样本中分离出的菌株。急性坏死性肺炎的发展以及IL-6、IL-8、IL-10和TNF-α表达的显著升高首次证明了食源性PVL阳性MSSA菌株对公众健康的重要性。
