Morriss-Kay G M, Tuckett F, Solursh M
Department of Human Anatomy, Oxford, UK.
J Embryol Exp Morphol. 1986 Nov;98:59-70.
Day 9 rat embryos (late presomite stage with cranial neural plate or very early neural folds) were cultured for various periods of time from 6-48 h in medium containing 20 TRU ml-1 Streptomyces hyaluronidase. Exposure to the enzyme resulted in considerable reduction of mesenchymal extracellular matrix. Access of the enzyme to the embryo was confirmed by alcian blue staining which indicated considerable reduction of extracellular and cell surface hyaluronate. Cranial neurulation was retarded, but not inhibited, and migration of both neural crest and primary mesenchyme cells occurred. In general, morphology was normal at 48 h. The major effect was on growth: embryos were smaller, with slightly reduced neuroepithelial cell number and greatly reduced mesenchymal cell number. Neuroepithelial cell cycle time was slightly prolonged, and that of the mesenchyme more than doubled. This differential effect on the growth rates of these two tissues reflects the normal distribution of hyaluronate, which is particularly abundant in the mesenchymal extracellular matrix.
第9天的大鼠胚胎（处于晚期体节前期，具有颅神经板或非常早期的神经褶）在含有20 TRU/ml透明质酸链霉菌酶的培养基中培养6至48小时不等。接触该酶导致间充质细胞外基质显著减少。通过阿尔辛蓝染色证实酶能够作用于胚胎，这表明细胞外和细胞表面的透明质酸盐显著减少。颅神经管形成受到阻碍，但未被抑制，神经嵴和原间充质细胞均发生迁移。总体而言，48小时时形态正常。主要影响在于生长：胚胎较小，神经上皮细胞数量略有减少，间充质细胞数量大幅减少。神经上皮细胞周期时间略有延长，间充质细胞周期时间则增加了一倍多。对这两种组织生长速率的这种差异影响反映了透明质酸盐的正常分布，其在间充质细胞外基质中特别丰富。
