Schoenwolf G C, Fisher M
J Embryol Exp Morphol. 1983 Feb;73:1-15.
Chick embryos at stages 8 to 9 were treated in ovo with Streptomyces hyaluronidase (SH) to determine whether neurulation occurs normally in embryos depleted of hyaluronic acid, a major component of the extracellular matrix. Open neural tube defects occurred in 60-94% (depending on the particular enzyme batch) of the embryos treated with SH and examined after an additional 24 h of incubation. Defects were confined mainly to the spinal cord. The neural folds underwent elevation in defective regions but failed to converge and fuse across the dorsal midline. The extracellular matrix of embryos treated with SH was depleted consistently, as determined with sections stained with Alcian blue. Control experiments were done to ensure that neural tube defects were not caused by non-specific protease contamination of SH, or by digestion products of hyaluronic acid. We propose several plausible and testable mechanisms through which the extracellular matrix might influence the complex developmental process of neurulation.
用透明质酸链霉菌（SH）对处于8至9期的鸡胚进行卵内处理，以确定在缺乏细胞外基质的主要成分透明质酸的胚胎中神经胚形成是否正常发生。在用SH处理并在额外孵育24小时后检查的胚胎中，60%-94%（取决于特定的酶批次）出现了开放性神经管缺陷。缺陷主要局限于脊髓。神经褶在缺陷区域隆起，但未能在背侧中线处会合和融合。用阿尔辛蓝染色的切片测定，经SH处理的胚胎的细胞外基质持续减少。进行了对照实验，以确保神经管缺陷不是由SH的非特异性蛋白酶污染或透明质酸的消化产物引起的。我们提出了几种合理且可测试的机制，通过这些机制细胞外基质可能会影响神经胚形成这一复杂的发育过程。
