Morris-Wiman J, Brinkley L L
Department of Anatomy and Cell Biology, Medical School, University of Michigan, Ann Arbor 48109.
Anat Rec. 1990 Mar;226(3):383-95. doi: 10.1002/ar.1092260316.
The mesenchyme of the elevating mesencephalic neural folds of the mouse is composed primarily of mesenchymal cells embedded in an hyaluronate-rich extracellular matrix. In this study we provide evidence that hyaluronate and mesenchymal expansion may play a role in neural fold elevation and closure. Spatial and temporal patterns of mesenchymal cell and hyaluronate distribution were analyzed during neural fold elevation and closure using the computer-assisted method of smoothed spatial averaging and established methods of image processing. Degree of fold elevation and fold shape changes were analyzed using standard morphometric measures. The results of these analyses defined five distinct stages in mesencephalic neural fold elevation and closure. Mesenchymal cells and hyaluronate were found in a non-random distribution within the neural fold and showed distinct patterns of distribution which could be correlated with specific stages in neural fold elevation. The results of these analyses suggested that the elevation of the mesencephalic neural folds is produced by the expansion of an hyaluronate-rich extracellular matrix in the central mesenchyme which under the direction of surrounding tissues pushes the folds mediad towards the dorsal midline.
小鼠中脑神经褶隆起处的间充质主要由嵌入富含透明质酸盐的细胞外基质中的间充质细胞组成。在本研究中，我们提供证据表明透明质酸盐和间充质扩张可能在神经褶隆起和闭合过程中发挥作用。在神经褶隆起和闭合过程中，使用平滑空间平均的计算机辅助方法和既定的图像处理方法，分析了间充质细胞和透明质酸盐分布的时空模式。使用标准形态测量方法分析了褶隆起程度和褶形状变化。这些分析结果确定了中脑神经褶隆起和闭合的五个不同阶段。发现间充质细胞和透明质酸盐在神经褶内呈非随机分布，并显示出与神经褶隆起的特定阶段相关的不同分布模式。这些分析结果表明，中脑神经褶的隆起是由中央间充质中富含透明质酸盐的细胞外基质扩张产生的，在周围组织的引导下，该基质将褶向中线方向推向背侧中线。
