Chrismadha Tjandra, Satya Awalina, Satya Ika Atman, Rosidah Rosidah, Satya Azalea Dyah Maysarah, Pangestuti Ratih, Harimawan Ardiyan, Setiadi Tjandra, Chew Kit Wayne, Show Pau Loke
Research Center for Limnology and Water Resources, National Research and Innovation Agency (BRIN), KST Soekarno, Jl. Raya Bogor-Jakarta km46, Cibinong, Bogor 16911, Indonesia.
Research Center for Computation, National Research and Innovation Agency (BRIN), Komplek Cisitu, Jl. Sangkuriang, Coblong, Bandung 40135, Indonesia.
Metabolites. 2022 Nov 30;12(12):1199. doi: 10.3390/metabo12121199.
Implementation of outdoor photobioreactors has been challenged by an extremely oversaturated daily peak of solar irradiance. This study aims to understand the role of column size and paranet shading as well as to investigate the most convenient light control in outdoor cyanobacterial culture. The photobioreactor (PBR) consisted of plastic columns with a diameter of 12.74 cm (PBRd-20) and 31.85 cm (PBRd-50) laid outdoors and inclined at 158.22° upwards against solar radiation, while paranet shading was provided at 0%, 50%, 70%, and 90% shading capacity. A semi-continuous culture of cyanobacterium was conducted for 6 weeks with weekly monitoring of the growth parameter as well as the proximate and pigments content, while the daily irradiance and culture maximum temperature were recorded. The result shows that the column diameter of 12.74 cm had a lethal risk of 44.7% and this decreased to 10.5% by widening the column diameter to 31.85 cm. This lethal risk can be eliminated by the application of a paranet at a 50% reduction level for the column diameter of 31.85 cm and a 70% reduction level for the column diameter of 12.74 cm. The highest culture productivity of 149.03 mg/(L·day) was achieved with a PBRd-20 with 50% shading treatment, but a PBRd-50 with 90% shading treatment led to an increase in the protein and phycocyanin content by 66.7% and 14.91%, respectively.
户外光生物反应器的应用受到每日太阳辐照度峰值极高且过饱和的挑战。本研究旨在了解柱体尺寸和遮阳网遮光的作用，并探究室外蓝藻培养中最便捷的光照控制方法。光生物反应器（PBR）由直径为12.74厘米（PBRd - 20）和31.85厘米（PBRd - 50）的塑料柱组成，放置在户外并向上倾斜158.22°以抵御太阳辐射，同时提供遮光能力为0%、50%、70%和90%的遮阳网遮光处理。对蓝藻进行了为期6周的半连续培养，每周监测生长参数以及近端成分和色素含量，同时记录每日辐照度和培养的最高温度。结果表明，直径为12.74厘米的柱体有44.7%的致死风险，将柱体直径扩大到31.85厘米时，该致死风险降至10.5%。对于直径为31.85厘米的柱体，应用遮光能力降低50%的遮阳网，对于直径为12.74厘米的柱体，应用遮光能力降低70%的遮阳网，可消除这种致死风险。在进行50%遮光处理的PBRd - 20中实现了最高培养生产力，为149.03毫克/（升·天），但进行90%遮光处理的PBRd - 50使蛋白质和藻蓝蛋白含量分别增加了66.7%和14.91%。
