Yang Xinyu, Zhao Xiaodan, Fu Daqi, Zhao Ying
School of Food and Health, Beijing Technology and Business University, Beijing 100048, China.
Laboratory of Fruit Biology, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Metabolites. 2022 Dec 19;12(12):1296. doi: 10.3390/metabo12121296.
Tomato is abundant in alkaloids, phenolic acids, and flavonoids; however, the effect of transcription factor NOR-like1 on these metabolites in tomato is unclear. We used a combination of widely targeted metabolomics and transcriptomics to analyze wild-type tomatoes and CR-NOR-like1 tomatoes. A total of 83 alkaloids, 85 phenolic acids, and 96 flavonoids were detected with significant changes. Combined with a KEGG enrichment analysis, we revealed 16 differentially expressed genes (DEGs) in alkaloid-related arginine and proline metabolism, 60 DEGs were identified in the phenolic acid-related phenylpropane biosynthesis, and 30 DEGs were identified in the flavonoid-related biosynthesis pathway. In addition, some highly correlated differential-expression genes with differential metabolites were further identified by correlation analysis. The present research provides a preliminary view of the effects of NOR-like1 transcription factor on alkaloid, phenolic acid, and flavonoid accumulation in tomatoes at different ripening stages based on widely targeted metabolomics and transcriptomics in plants, laying the foundation for extending fruit longevity and shelf life as well as cultivating stress-resistant plants.
番茄富含生物碱、酚酸和黄酮类化合物；然而，转录因子NOR-like1对番茄中这些代谢产物的影响尚不清楚。我们结合广泛靶向代谢组学和转录组学来分析野生型番茄和CR-NOR-like1番茄。共检测到83种生物碱、85种酚酸和96种黄酮类化合物有显著变化。结合KEGG富集分析，我们发现在生物碱相关的精氨酸和脯氨酸代谢中有16个差异表达基因（DEGs），在酚酸相关的苯丙烷生物合成中有60个DEGs，在黄酮类相关生物合成途径中有30个DEGs。此外，通过相关性分析进一步鉴定了一些与差异代谢产物高度相关的差异表达基因。本研究基于植物广泛靶向代谢组学和转录组学，初步揭示了NOR-like1转录因子对不同成熟阶段番茄中生物碱、酚酸和黄酮类化合物积累的影响，为延长果实寿命和货架期以及培育抗逆植物奠定了基础。
