AbdelKhalek Ahmed, Narayanan Sanjeev K
Department of Comparative Pathobiology, Purdue University, 625 Harrison Street, West Lafayette, IN 47907, USA.
Purdue Institute of Inflammation, Immunology, and Infectious Disease, West Lafayette, IN 47907, USA.
Microorganisms. 2022 Nov 30;10(12):2380. doi: 10.3390/microorganisms10122380.
causes the highest number of nosocomial infections. Currently, treatment options for infection (CDI) are very limited, resulting in poor treatment outcomes and high recurrence rates. Although the disease caused by CDI is inflammatory in nature, the role of inflammation in the development of CDI symptoms is contradictory and not completely understood. Hence, the use of anti-inflammatory medication is debatable in CDI. In the current study, we evaluated the genetic and microbiome profiles of mice after infection with . These mice were categorized based on the severity of CDI and the results were viewed accordingly. Our results indicate that certain genes are upregulated in severe CDI more than in the moderate case. These include oncostatin-M matrix metalloprotease 8 triggering receptor expressed on myeloid cells 1 ), and dual oxidase 2 . We also investigated the microbiome composition of CDI mice before and after infecting with . The results show that abundance is not indicative of diseases severity. Certain bacterial species (e.g., ) were enriched while others (e.g., ) were absent in severe CDI. This study identifies novel inflammatory pathways and bacterial species with a potential role in determining the severity of CDI.
导致医院感染数量最多。目前，艰难梭菌感染（CDI）的治疗选择非常有限，导致治疗效果不佳且复发率高。尽管CDI引起的疾病本质上是炎症性的，但炎症在CDI症状发展中的作用是矛盾的，尚未完全了解。因此，在CDI中使用抗炎药物存在争议。在当前的研究中，我们评估了感染[具体病原体未提及]后小鼠的基因和微生物组概况。这些小鼠根据CDI的严重程度进行分类，并相应地查看结果。我们的结果表明，某些基因在严重CDI中比在中度病例中上调得更多。这些基因包括抑瘤素-M、基质金属蛋白酶8、髓样细胞表达的触发受体1和双氧化酶2。我们还研究了感染[具体病原体未提及]前后CDI小鼠的微生物组组成。结果表明，[具体细菌未提及]的丰度并不表明疾病的严重程度。某些细菌物种（例如[具体细菌未提及]）在严重CDI中富集，而其他一些细菌物种（例如[具体细菌未提及]）则不存在。这项研究确定了在决定CDI严重程度方面具有潜在作用的新的炎症途径和细菌物种。
