Barra Lucia, Sardo Angela, Moros Maria, Smerilli Arianna, Chiaiese Pasquale, Percopo Isabella, Cavalletti Elena, Castro-Hinojosa Christian, Balzano Sergio
Department of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Contrada Torre Spaccata, 87071 Amendolara, Italy.
Department of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Via Acton 55, 80133 Naples, Italy.
Microorganisms. 2022 Dec 10;10(12):2445. doi: 10.3390/microorganisms10122445.
Heavy metals (HMs) can induce both chronic and acute harmful effects on marine and freshwater biota. The environmental impact of HMs in freshwater, seawater, soil, and wastewater can be limited using microbes, including microalgae, that are able to remove metals from environmental matrices. Indeed, they can passively adsorb and actively accumulate these persistent pollutants within their organelles, limiting their detrimental effects on cellular metabolism. The Sarno River is a 30 km long freshwater stream located in Southern Italy, polluted by partially untreated municipal, agricultural, and industrial wastewaters. In spite of this, microalgal cultures from Sarno River or Sarno River Mouth have never been established. In the present study, we isolated a green algal strain from the Sarno River Mouth and determined its ability to grow in polluted seawater containing different concentrations of cadmium, lead, or zinc. This strain was found to be able to accumulate these elements within its biomass in a dose-dependent manner. Growth inhibition experiments confirm the relatively low toxicity of Cd and Pb below 50 µM, while algal growth was seriously affected in Zn-amended media. To the best of our knowledge, this is the first study focused on the ability of microalgae from Sarno River Mouth to tolerate and uptake HMs.
重金属（HMs）可对海洋和淡水生物群造成慢性和急性有害影响。利用包括微藻在内的微生物可以限制重金属在淡水、海水、土壤和废水中对环境的影响，这些微生物能够从环境基质中去除金属。事实上，它们可以被动吸附并主动在细胞器内积累这些持久性污染物，从而限制其对细胞代谢的有害影响。萨尔诺河是一条位于意大利南部的淡水溪流，长30公里，受到部分未经处理的城市、农业和工业废水的污染。尽管如此，从未建立过来自萨尔诺河或萨尔诺河河口的微藻培养物。在本研究中，我们从萨尔诺河河口分离出一种绿藻菌株，并测定了其在含有不同浓度镉、铅或锌的污染海水中生长的能力。发现该菌株能够以剂量依赖的方式在其生物量中积累这些元素。生长抑制实验证实，镉和铅在50µM以下时毒性相对较低，而在添加锌的培养基中藻类生长受到严重影响。据我们所知，这是第一项关注萨尔诺河河口微藻耐受和吸收重金属能力的研究。
