Department of Physics and Astronomy, Theodore Jorgensen Hall, 855 North 16th Street, University of Nebraska-Lincoln, Lincoln, NE 68588-0299, USA.
Department of Chemistry, Hamilton Hall, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Molecules. 2022 Dec 16;27(24):8960. doi: 10.3390/molecules27248960.
The coordination chemistry of uranyl ions with surface immobilized peptides was studied using X-ray photoemission spectroscopy (XPS). All the peptides in the study were modified using a six-carbon alkanethiol as a linker on a gold substrate with methylene blue as the redox label. The X-ray photoemission spectra reveal that each modified peptide interacts differently with the uranyl ion. For all the modified peptides, the XPS spectra were taken in both the absence and presence of the uranium, and their comparison reveals that the interaction depends on the chemical group present in the peptides. The XPS results show that, among all the modified peptides in the current study, the (arginine) (R9) modified peptide showed the largest response to uranium. In the order of response to uranium, the second largest response was shown by the modified (arginine) (R6) peptide followed by the modified (lysine) (K6) peptide. Other modified peptides, (alanine) (A6), (glutamic acid) (E6) and (serine) (S6), did not show any response to uranium.
采用 X 射线光电子能谱（XPS）研究了铀酰离子与表面固定化肽的配位化学。研究中所有的肽都通过在金基底上使用六碳链烷硫醇作为连接体，并以亚甲蓝作为氧化还原标记来修饰。X 射线光电子能谱揭示了每个修饰肽与铀酰离子的相互作用方式不同。对于所有修饰的肽，在不存在和存在铀的情况下都进行了 XPS 光谱测量，它们的比较表明相互作用取决于肽中存在的化学基团。XPS 结果表明，在当前研究的所有修饰肽中，（精氨酸）（R9）修饰肽对铀的响应最大。按对铀的响应顺序，其次是（精氨酸）（R6）修饰肽，然后是（赖氨酸）（K6）修饰肽。其他修饰肽，（丙氨酸）（A6）、（谷氨酸）（E6）和（丝氨酸）（S6），对铀没有任何响应。
