Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Department of Biology, College of Science, University of Jeddah, Jeddah 21493, Saudi Arabia.
Nutrients. 2022 Dec 8;14(24):5240. doi: 10.3390/nu14245240.
Macrophages are one of the most important cells in the immune system. They act as links between innate and adaptive immunities. In this study, the aim was to examine thymoquinone effects on the immunological properties of different macrophages. Peripheral blood mononuclear cells were isolated from blood from healthy volunteers by negative selection of monocytes that had been cultured for seven days to differentiate into macrophages. Cells were cultured with or without the presence of thymoquinone (TQ), which was used in two different concentrations (50 μg/mL and 100 μg/mL. Cluster of differentiation 80 (CD80), cluster of differentiation 86 (CD86), and human leukocyte antigen DR isotype (HLA-DR) were measured by flow cytometry, and the secretion of interferon gamma (IFN-γ) and tumour necrosis factor alpha (TNF-α) was measured. Cells were also tested for their phagocytosis abilities. The data showed that the expression of HLA-DR was significantly higher in cells treated with 100 μL/mL TQ. In addition, IFN-γ concentration increased in the 100 μg/mL TQ-treated cells. The macrophage phagocytosis results showed a significant difference in 50 μg/mL TQ-treated cells compared to the controls. TQ may enhance the immunological properties of macrophages during the early stages of innate immunity by activating phagocytosis ability and by increasing the expression of HLA-DR and the secretion of IFN-γ, which may enhance the antigen-presentation capabilities of macrophages.
巨噬细胞是免疫系统中最重要的细胞之一。它们充当固有免疫和适应性免疫之间的联系。在这项研究中，目的是研究胸腺醌对不同巨噬细胞免疫特性的影响。外周血单核细胞通过阴性选择从健康志愿者的血液中分离出来，这些单核细胞经过七天的培养分化为巨噬细胞。将细胞与或不与胸腺醌（TQ）一起培养，TQ 以两种不同浓度（50μg/mL 和 100μg/mL）使用。通过流式细胞术测量分化群 80（CD80）、分化群 86（CD86）和人类白细胞抗原 DR 同种型（HLA-DR）的表达，测量干扰素γ（IFN-γ）和肿瘤坏死因子α（TNF-α）的分泌。还测试了细胞的吞噬能力。数据显示，用 100μL/mL TQ 处理的细胞中 HLA-DR 的表达明显更高。此外，用 100μg/mL TQ 处理的细胞中 IFN-γ浓度增加。与对照组相比，50μg/mL TQ 处理的细胞中巨噬细胞吞噬作用的结果显示出显著差异。TQ 可能通过激活吞噬作用能力、增加 HLA-DR 的表达和 IFN-γ的分泌，从而增强巨噬细胞的抗原呈递能力，从而在固有免疫的早期阶段增强巨噬细胞的免疫特性。
