Guilhon Carolina Carvalho, Minho Alan Silva, Pouliot Marc, Boylan Fabio, Fernandes Patricia Dias
Laboratório de Farmacologia da Dor e da Inflamação, Programa de Pesquisa em Descoberta de Fármacos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
Centre de Recherche du CHU de Québec, Université Laval, Québec, QC G1V 4G2, Canada.
Pharmaceuticals (Basel). 2022 Nov 24;15(12):1458. doi: 10.3390/ph15121458.
The ethanol extract (EE) prepared from the leaves of , and its fraction in ethyl acetate (fEA) were evaluated concerning their capacity to reduce inflammation in different experimental models. fEA was also studied concerning its chemical constituents. EE and fEA were assayed for their anti-inflammatory potential, using formalin-induced licking behavior and carrageenan-induced inflammation into the subcutaneous air pouch (SAP) models. Reduction in polymorphonuclear cells (PMN) activation was performed in freshly isolated PMN. Chromatographic analysis of fEA was performed by HPLC-DAD. Hispiduloside was isolated as the main constituent in fEA, and its quantity was estimated to be 39.3% in fEA. EE (30 mg/kg) significantly reduced the second phase of formalin-induced licking. fEA demonstrated a reduction in leukocyte migration into the SAP. EE and fEA drastically reduced cytokines (TNF-α, IL-1β, and IFN-γ), nitric oxide (NO) production, in vitro PMN migration induced by C5a and IL-8, and TNF-α and IL-1β gene expression. Taken together, our data indicate that either ethanol extract or its fEA fraction from leaves of present an anti-inflammatory effect, contributing to the pharmacological and chemical knowledge of this species and confirming the rationale behind its traditional use.
从[植物名称]叶子中制备的乙醇提取物（EE）及其乙酸乙酯馏分（fEA），在不同实验模型中对其减轻炎症的能力进行了评估。还对fEA的化学成分进行了研究。使用福尔马林诱导的舔舐行为和角叉菜胶诱导的皮下气囊（SAP）炎症模型，对EE和fEA的抗炎潜力进行了测定。在新鲜分离的多形核细胞（PMN）中进行PMN活化的降低实验。通过HPLC-DAD对fEA进行色谱分析。分离出hispiduloside作为fEA中的主要成分，其含量估计在fEA中为39.3%。EE（30mg/kg）显著降低了福尔马林诱导舔舐的第二阶段。fEA显示白细胞向SAP中的迁移减少。EE和fEA极大地降低了细胞因子（TNF-α、IL-1β和IFN-γ）、一氧化氮（NO）的产生、C5a和IL-8诱导的体外PMN迁移以及TNF-α和IL-1β基因表达。综上所述，我们的数据表明，来自[植物名称]叶子的乙醇提取物或其fEA馏分具有抗炎作用，有助于丰富该物种的药理和化学知识，并证实了其传统用途背后的原理。
