Bustos-Gómez Chrystyan Iván, Gasca-Martínez Deisy, Yáñez-Barrientos Eunice, Hidalgo-Figueroa Sergio, Gonzalez-Rivera Maria L, Barragan-Galvez Juan Carlos, Zapata-Morales Juan Ramón, Isiordia-Espinoza Mario, Corrales-Escobosa Alma Rosa, Alonso-Castro Angel Josabad
Maestría en Ciencias Farmacéuticas, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato 36200, Mexico.
Unidad de Análisis Conductual, Instituto de Neurobiología, Campus UNAM-Juriquilla, Juriquilla 76230, Mexico.
Pharmaceuticals (Basel). 2022 Dec 18;15(12):1580. doi: 10.3390/ph15121580.
(Kunth) Britten & Baker f (Malvaceae) is used for the folk treatment of mood disorders. bark was extracted in ethanol, and the extract (CAE) was chemically standardized using gas chromatography-mass spectrometry (GC-MS). This study evaluated the effects of CAE (10-100 mg/kg p.o.) on anxiolytic-like activity, sedation, locomotor activity, depression-like activity, and spatial working memory using in vivo rodent models. A possible mechanism for the anxiolytic-like and antidepressant-like actions induced by CAE was assessed using neurotransmission pathway inhibitors. Myristic acid was one of the compounds found in CAE using GC-MS. This study also evaluated the anxiolytic-like activity and the sedative actions of myristic acid and assessed a possible mechanism of action using neurotransmission pathway inhibitors and an in silico analysis. CAE elicited anxiolytic-like activity and antidepressant-like effects (ED = 57 mg/kg). CAE (10-100 mg/kg) did not affect locomotor coordination or induce sedation. The anxiolytic-like and antidepressant-like actions of CAE were reverted by prazosin, suggesting a possible participation of the noradrenergic system. The anxiolytic-like activity of myristic acid was reverted by the co-administration of prazosin and partially reverted by ketanserin. The docking study revealed that myristic acid can form favorable interactions within 5-HT2A and α1A-adrenoreceptor binding pockets.
（昆斯）布里顿氏棉葵（锦葵科）被用于民间治疗情绪障碍。其树皮用乙醇提取，提取物（CAE）通过气相色谱 - 质谱联用（GC - MS）进行化学标准化。本研究使用体内啮齿动物模型评估了CAE（口服10 - 100mg/kg）对抗焦虑样活性、镇静作用、运动活性、抑郁样活性和空间工作记忆的影响。使用神经传递途径抑制剂评估了CAE诱导抗焦虑样和抗抑郁样作用的可能机制。肉豆蔻酸是通过GC - MS在CAE中发现的化合物之一。本研究还评估了肉豆蔻酸的抗焦虑样活性和镇静作用，并使用神经传递途径抑制剂和计算机模拟分析评估了可能的作用机制。CAE产生了抗焦虑样活性和抗抑郁样作用（ED = 57mg/kg）。CAE（10 - 100mg/kg）不影响运动协调性或诱导镇静。哌唑嗪可逆转CAE的抗焦虑样和抗抑郁样作用，提示去甲肾上腺素能系统可能参与其中。肉豆蔻酸的抗焦虑样活性在与哌唑嗪共同给药时被逆转，在与酮色林共同给药时部分被逆转。对接研究表明，肉豆蔻酸可在5 - HT2A和α1A - 肾上腺素能受体结合口袋内形成有利的相互作用。
