Campanholi Katieli da Silva Souza, da Silva Junior Ranulfo Combuca, Gonçalves Renato Sonchini, de Oliveira Mariana Carla, Pozza Magali Soares Dos Santos, Leite Angela Tiago, da Silva Leandro Herculano, Malacarne Luis Carlos, Bruschi Marcos Luciano, Castilha Leandro Dalcin, Dos Santos Tatiana Carlesso, Caetano Wilker
Chemistry Department, State University of Maringá, Maringá 87020-900, PR, Brazil.
Laboratory of Chemistry of Natural Products, Department of Chemistry, Center for Exact Sciences and Technology, Federal University of Maranhão, São Luís 65080-805, MA, Brazil.
Pharmaceutics. 2022 Nov 24;14(12):2580. doi: 10.3390/pharmaceutics14122580.
Chronic wound healing represents an impactful financial burden on healthcare systems. In this context, the use of natural products as an alternative therapy reduces costs and maintains effectiveness. Phytotherapeutic gels applied in photodynamic therapy (PDT) have been developed to act as topical healing medicines and antibiotics. The bioactive system is composed of sp. (source of chlorophylls) and oil microdroplets, both incorporated into a polymeric blend constituted by carrageenan (car) and F127 copolymer, constituting a system in which all components are bioactive agents. The flow behavior and viscoelasticity of the formulations were investigated. The photodynamic activity was accessed from studies of the inactivation of bacteria, the main pathogen of hospital relevance. Furthermore, in vivo studies were conducted using eighteen rabbits with dermatitis (grade III and IV) in both paws. The gels showed significant antibiotic potential in vitro, eliminating up to 100% of colonies in the presence or absence of light. The car reduced 41% of the viable cells; however, its benefits were enhanced by adding chlorophyll and copaiba oil. The animals treated with the phytotherapeutic medicine showed a reduction in lesion size, with healing and re-epithelialization verified in the histological analyses. The animals submitted to PDT displayed noticeable improvement, indicating this therapy's viability for ulcerative and infected wounds. This behavior was not observed in the iodine control treatment, which worsened the animals' condition. Therefore, gel formulations were a viable alternative for future pharmaceutical applications, aiming at topical healing.
慢性伤口愈合给医疗系统带来了沉重的经济负担。在这种情况下，使用天然产品作为替代疗法可降低成本并保持疗效。已开发出用于光动力疗法（PDT）的植物治疗凝胶，用作局部愈合药物和抗生素。该生物活性体系由绿藻属（叶绿素来源）和 Copaiba 油微滴组成，二者均被纳入由角叉菜胶（car）和 F127 共聚物构成的聚合物共混物中，形成一个所有组分均为生物活性剂的体系。研究了制剂的流动行为和粘弹性。通过对医院相关主要病原体金黄色葡萄球菌的灭活研究来评估光动力活性。此外，使用 18 只双爪患有 III 级和 IV 级皮炎的兔子进行了体内研究。凝胶在体外显示出显著的抗生素潜力，在有光或无光的情况下可消除高达 100%的金黄色葡萄球菌菌落。角叉菜胶减少了 41%的活细胞；然而，添加叶绿素和 Copaiba 油增强了其效果。用植物治疗药物治疗的动物病变大小减小，组织学分析证实有愈合和重新上皮化现象。接受 PDT 治疗的动物有明显改善，表明该疗法对溃疡性和感染性伤口具有可行性。在碘对照治疗中未观察到这种情况，碘对照治疗使动物病情恶化。因此，凝胶制剂是未来用于局部愈合的药物应用的可行替代方案。
