Cabahug Raisa Aone M, Tran My Khanh Thi Ha, Ahn Yun-Jae, Hwang Yoon-Jung
Plant Genetics and Breeding Institute, Sahmyook University, Seoul 01795, Republic of Korea.
Department of Convergence Science, Sahmyook University, Seoul 01795, Republic of Korea.
Plants (Basel). 2022 Dec 7;11(24):3420. doi: 10.3390/plants11243420.
Mutation breeding has produced promising results, with exceptional attributes including pest/disease and environmental tolerance and desirable ornamental traits. Among the tools used in mutation breeding, chemical mutation is the most inexpensive way to develop novel plants. Succulents have gained popularity with high market demand because they require minimal watering and have plastic-like visuals. Ornamental succulents with rare leaf morphologies are costly. An LD study was conducted beforehand to determine the survival rates of colchicine-treated 'Peerless'. Mutants in the first generation (MV) were identified and analyzed. Determining whether mutagenic characteristics are carried to the subsequent generation (MV) is a key component in breeding programs. Additional investigation was performed by producing MV plants through vegetative propagation to determine mutagenic retention. For MV, mutants exhibited shortened leaves, increased leaf width and thickness, and fewer leaves, which significantly differed from the control, indicating compactness, wider leaf apex, and varying leaf color. To confirm the mutations, stomatal analysis was conducted, wherein there was a decrease in density and an increase in stomatal size. Likewise, chromosome counting and flow cytometry analysis confirmed the induction of polyploidization. Colchicine induction to develop new cultivars with novel phenotypic and cytogenetic characters is suitable for ornamental succulents.
诱变育种已取得了喜人的成果，其优异特性包括对病虫害和环境的耐受性以及理想的观赏性状。在诱变育种所使用的工具中，化学诱变是培育新型植物最经济的方法。多肉植物因需水量极少且具有类似塑料的外观而在市场上广受欢迎，需求旺盛。具有罕见叶片形态的观赏多肉植物价格昂贵。事先进行了一项致死剂量（LD）研究，以确定秋水仙碱处理的“无双”品种的存活率。对第一代突变体（MV）进行了鉴定和分析。确定诱变特性是否会遗传至后代（MV）是育种计划的关键组成部分。通过营养繁殖培育MV植株以确定诱变保留情况，进而进行了额外的研究。对于MV，突变体表现出叶片缩短、叶宽和叶厚增加以及叶片数量减少的情况，这与对照有显著差异，表明其紧凑性、叶尖更宽以及叶片颜色各异。为了确认突变，进行了气孔分析，结果显示气孔密度降低且气孔大小增加。同样，染色体计数和流式细胞术分析证实了多倍体化的诱导。秋水仙碱诱导培育具有新表型和细胞遗传学特征的新品种适用于观赏多肉植物。
