Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130, China.
Department of Biology, Saint Mary's University, Halifax, NS, B3H3C3, Canada.
Sci Rep. 2017 Aug 17;7(1):8503. doi: 10.1038/s41598-017-08391-2.
Colchicine was commonly used to artificially double chromosomes while the transcriptome changes in colchicine treated plants has rarely been characterized. To understand the molecular mechanism of colchicine on chromosome doubling, we characterized transcriptome data of diploid orchardgrass root after colchicine treatment. Our results showed that 3381 of differentially expressed genes (DEGs) were mainly affected by water stress, 1258 DEGs that were expressed significantly in sample DacR5tr but not in DacR5ck were considered to be mainly affected by colchicine and combination of water and colchicine. These DEGs mainly regulated by colchicine were enriched to gene ontology (GO) accessions of cation binding, catalytic activity, membrane and transporter activity, and enriched to Kyoto Encyclopedia of Genes and Genome (KEGG) pathways of phenylpropanoid biosynthesis, phenylalanine metabolism, plant hormone signal transduction and starch and sucrose metabolism. Genes related to microtubule, spindle, chromosomal kinetochore, vesicle, cellulose and processes of cytoplasm movement, chromatid segregation, membrane and cell wall development were inhibited by colchicine. Our results revealed that colchicine restrained the microtubules and inhibited gene expression of cytokinesis, which might slow down the cell activity, delay the cell into anaerobic respiration, resulting in apoptosis at late stage, and relieving of waterlogging.
秋水仙素常用于人为地使染色体加倍，而秋水仙素处理植物的转录组变化很少被描述。为了了解秋水仙素在染色体加倍过程中的分子机制，我们对秋水仙素处理的二倍体草地早熟禾根的转录组数据进行了特征描述。结果表明，3381 个差异表达基因（DEGs）主要受水分胁迫影响，1258 个在 DacR5tr 样本中表达显著而在 DacR5ck 中不表达的 DEGs 被认为主要受秋水仙素和水分与秋水仙素的共同作用影响。这些主要受秋水仙素调控的 DEGs 被富集到阳离子结合、催化活性、膜和转运活性的基因本体 (GO) 条目，以及苯丙烷生物合成、苯丙氨酸代谢、植物激素信号转导和淀粉和蔗糖代谢的京都基因与基因组百科全书 (KEGG) 途径。与微管、纺锤体、染色体动粒、囊泡、纤维素和细胞质运动、染色单体分离、膜和细胞壁发育过程相关的基因受到秋水仙素的抑制。我们的结果表明，秋水仙素抑制了微管的形成，抑制了胞质分裂的基因表达，这可能会减缓细胞的活性，延迟细胞进入无氧呼吸，导致后期细胞凋亡，从而缓解涝害。
