El-Nashar Y I, Ammar M H
Plant Production Department, College of Food and Agriculture Sciences, King Saud University, King Saudi Arabia.
Ornamental Plants and Landscape Gardening Research Department, Horticultural Research Institute, Al-Montaza Garden, Giza, Egypt.
Genet Mol Res. 2016 Apr 26;15(2):gmr7745. doi: 10.4238/gmr.15027745.
Six different colchicine concentrations: 0, 400, 800, 1200, 1600, and 2000 ppm, in combination with four soaking time treatments (1, 2, 3, and 4 h), were selected to assess the effects on germination, vegetative growth, and flower yield components in calendula plants. The molecular diversity among the treatments was assessed using ten SRAP marker combinations. Seed soaking in colchicine significantly enhanced both the fresh and the dry shoot and root masses, flowering date, number of flowers per plant, and flower diameter. At 1200-ppm colchicine combined with a 4-h soaking time, a superior effect on seed germination was observed, whereas 800 ppm for 4 h produced the highest number of flowers and the largest flower diameter. The earliest flowering time was found at 800 ppm combined with a short soaking time (1 h), while the 4-h soaking time with 800 ppm, is recommended for growing calendula outdoors, since it enhances flower development. At the molecular level, 752 fragments were successfully amplified using the SRAP primers, with 280 genetic loci found throughout the calendula genome. The polymorphism percentage ranged from 79 to 100% and the polymorphic information content (PIC) values ranged between 0.85 and 0.97. The high number of detected loci and PIC values suggests a great power of SRAP markers in detecting mutant molecular diversity. Our results clearly show the existence of genetic variation among colchicine treated calendula plants and the clustering of the studied mutants was concordant with the colchicine concentration used.
0、400、800、1200、1600和2000 ppm，并结合四种浸泡时间处理（1、2、3和4小时），以评估其对金盏花植株发芽、营养生长和花产量构成因素的影响。使用十种SRAP标记组合评估各处理之间的分子多样性。用秋水仙碱浸泡种子显著提高了地上部和根部的鲜质量和干质量、开花日期、单株花数和花直径。在秋水仙碱浓度为1200 ppm并浸泡4小时时，观察到对种子发芽有较好的效果，而800 ppm浸泡4小时则产生了最多的花数和最大的花直径。最早的开花时间出现在800 ppm并浸泡时间较短（1小时）时，而对于户外种植金盏花，建议800 ppm浸泡4小时，因为这能促进花的发育。在分子水平上，使用SRAP引物成功扩增出752个片段，在整个金盏花基因组中发现了280个基因位点。多态性百分比在79%至100%之间，多态信息含量（PIC）值在0.85至0.97之间。检测到的基因位点数量众多以及PIC值表明SRAP标记在检测突变分子多样性方面具有强大的能力。我们的结果清楚地表明，经秋水仙碱处理的金盏花植株之间存在遗传变异，并且所研究突变体的聚类与所用秋水仙碱浓度一致。
