Núñez-Lillo Gerardo, Pérez-Reyes Wellasmin, Riveros Anibal, Lillo-Carmona Victoria, Rothkegel Karin, Álvarez José Miguel, Blanco-Herrera Francisca, Pedreschi Romina, Campos-Vargas Reinaldo, Meneses Claudio
Escuela de Agronomía, Facultad de Ciencias Agronómicas y de los Alimentos, Pontificia Universidad Católica de Valparaíso, Quillota 2260000, Chile.
Centro de Biotecnología Vegetal, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370186, Chile.
Plants (Basel). 2022 Dec 12;11(24):3473. doi: 10.3390/plants11243473.
Harvest date is a critical parameter for producers and consumers regarding agro-industrial performance. It involves a pleiotropic effect controlling the development of other fruit quality traits through finely controlling regulatory mechanisms. Fruit ripening is a process in which various signals and biological events co-occur and are regulated by hormone signaling that produces the accumulation/degradation of multiple compounds. However, the regulatory mechanisms that control the hormone signaling involved in fruit development and ripening are still unclear. To investigate the issue, we used individuals with early, middle and late harvest dates from a peach segregating population to identify regulatory candidate genes controlling fruit quality traits at the harvest stage and validate them in contrasting peach varieties for this trait. We identified 467 and 654 differentially expressed genes for early and late harvest through a transcriptomic approach. In addition, using the Arabidopsis DAP-seq database and network analysis, six transcription factors were selected. Our results suggest significant hormonal balance and cell wall composition/structure differences between early and late harvest samples. Thus, we propose that higher expression levels of the transcription factors , and in early harvest individuals would induce the expression of genes associated with the jasmonic acid pathway, photosynthesis and gibberellins inhibition. While on the other hand, the high expression levels of , and in late harvest individuals would promote the induction of genes associated with abscisic acid biosynthesis, auxins and cell wall remodeling.
收获日期对于生产者和消费者而言，是关乎农产品加工业表现的关键参数。它涉及一种多效性效应，通过精细调控调控机制来控制其他果实品质性状的发育。果实成熟是一个各种信号和生物学事件同时发生且受激素信号调控的过程，激素信号会导致多种化合物的积累/降解。然而，控制果实发育和成熟过程中激素信号的调控机制仍不清楚。为了研究这个问题，我们利用一个桃分离群体中收获日期早、中、晚的个体，来鉴定在收获阶段控制果实品质性状的调控候选基因，并在该性状表现不同的桃品种中对其进行验证。我们通过转录组学方法鉴定出了467个和654个分别在早收和晚收时差异表达的基因。此外，利用拟南芥DAP-seq数据库和网络分析，挑选出了6个转录因子。我们的结果表明，早收和晚收样本之间存在显著的激素平衡以及细胞壁组成/结构差异。因此，我们提出，早收个体中转录因子 、 和 的较高表达水平会诱导与茉莉酸途径、光合作用和赤霉素抑制相关的基因表达。而另一方面，晚收个体中 、 和 的高表达水平会促进与脱落酸生物合成、生长素和细胞壁重塑相关基因的诱导表达。
