Núñez-Lillo Gerardo, Ulloa-Zepeda Lissette, Pavez Catalina, Riveros Anibal, Blanco-Herrera Francisca, Campos-Vargas Reinaldo, Pedreschi Romina, Meneses Claudio
Escuela de Agronomía, Facultad de Ciencias Agronómicas y de los Alimentos, Pontificia Universidad Católica de Valparaíso, Calle San Francisco s/n, La Palma, Quillota 2260000, Chile.
Centro de Biotecnología Vegetal, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370186, Chile.
Plants (Basel). 2021 Nov 5;10(11):2380. doi: 10.3390/plants10112380.
Fruit development is a complex process that involves the interplay of cell division, expansion, and differentiation. As a model to study fruit development, nectarines incapable of ripening were described as slow ripening. Slow ripening fruits remained firm and exhibited no rise in CO or ethylene production rates for one month or more at 20 °C. Different studies suggest that this trait is controlled by a single gene (). Transcriptome analysis between normal and slow ripening fruits showed a total of 157, 269, 976, and 5.224 differentially expressed genes in each fruit developmental stage analyzed (T1, T2, T3, and T7, respectively), and no expression of was found in the slow ripening individuals. Using this transcriptomic information, we identified a correlation of with auxin-related genes and two genes associated with terpene biosynthesis. On the other hand, significant differences were observed in hormonal biosynthetic pathways during fruit development between the normal and slow ripening individuals (gibberellin, ethylene, jasmonic acid and abscisic acid). These results suggest that the absence of by the direct or indirect expression control of auxins or terpene-related genes prevents normal peach fruit development.
果实发育是一个复杂的过程，涉及细胞分裂、扩展和分化之间的相互作用。作为研究果实发育的模型，无法成熟的油桃被描述为成熟缓慢。成熟缓慢的果实保持坚硬，在20℃下一个月或更长时间内二氧化碳或乙烯生成速率没有增加。不同研究表明，该性状由单个基因控制。对正常和成熟缓慢果实的转录组分析显示，在分析的每个果实发育阶段（分别为T1、T2、T3和T7），共有157、269、976和5224个差异表达基因，并且在成熟缓慢的个体中未发现 的表达。利用这些转录组信息，我们确定了 与生长素相关基因以及与萜类生物合成相关的两个基因之间的相关性。另一方面，在正常和成熟缓慢的个体之间（赤霉素、乙烯、茉莉酸和脱落酸），果实发育过程中的激素生物合成途径存在显著差异。这些结果表明，生长素或萜类相关基因的直接或间接表达控制导致 的缺失，从而阻止了普通桃果实的正常发育。
