Postiglione Alessia, Prigioniero Antonello, Zuzolo Daniela, Tartaglia Maria, Scarano Pierpaolo, Maisto Maria, Ranauda Maria Antonietta, Sciarrillo Rosaria, Thijs Sofie, Vangronsveld Jaco, Guarino Carmine
Department of Science and Technology, University of Sannio, via de Sanctis snc, 82100 Benevento, Italy.
Environmental Biology, Centre for Environmental Sciences, Hasselt University, Agoralaan, Building D, 3590 Diepenbeek, Belgium.
Plants (Basel). 2022 Dec 15;11(24):3528. doi: 10.3390/plants11243528.
The intra- and interdomain phyllosphere microbiome features of L. in a Mediterranean context is reported. We hypothesized that the main driver of the phyllosphere microbiome might be the season and that atmospheric pollutants might have a co-effect. Hence, we investigated the composition of epiphytic bacteria and fungi of leaves sampled in urban and natural areas (in Southern Italy) in summer and winter, using microscopy and metagenomic analysis. To assess possible co-effects on the composition of the phyllosphere microbiome, concentrations of particulate matter and polycyclic aromatic hydrocarbons (PAHs) were determined from sampled leaves. We found that environmental factors had a significative influence on the phyllosphere biodiversity, altering the taxa relative abundances. Ascomycota and Firmicutes were higher in summer and in urban areas, whereas a significant increase in Proteobacteria was observed in the winter season, with higher abundance in natural areas. Network analysis suggested that OTUs belonging to Acidobacteria, Cytophagia, unkn. Firmicutes(p), Actinobacteria are keystone of the phyllosphere microbiome. In addition, 83 genes coding for 5 enzymes involved in PAH degradation pathways were identified. Given that the phyllosphere microbiome can be considered an extension of the ecosystem services offered by trees, our results can be exploited in the framework of Next-Generation Biomonitoring.
报道了地中海地区内域和域间叶际微生物群的特征。我们假设叶际微生物群的主要驱动因素可能是季节，并且大气污染物可能具有协同效应。因此，我们使用显微镜和宏基因组分析，研究了夏季和冬季在城市和自然区域（意大利南部）采集的叶片上附生细菌和真菌的组成。为了评估对叶际微生物群组成可能的协同效应，测定了从采集叶片中颗粒物和多环芳烃（PAHs）的浓度。我们发现环境因素对叶际生物多样性有显著影响，改变了分类群的相对丰度。子囊菌门和厚壁菌门在夏季和城市地区含量较高，而在冬季观察到变形菌门显著增加，在自然区域含量更高。网络分析表明，属于酸杆菌门、噬纤维菌属、未知厚壁菌门（p）、放线菌门的操作分类单元是叶际微生物群的关键物种。此外，还鉴定出了83个编码参与多环芳烃降解途径的5种酶的基因。鉴于叶际微生物群可被视为树木提供的生态系统服务的延伸，我们的研究结果可在下一代生物监测框架中加以利用。
