Grove D I, Warton A, Northern C, Papadimitriou J M
Department of Medicine, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands.
J Parasitol. 1987 Oct;73(5):1030-4.
Previous indications using radiolabelled larvae that Strongyloides ratti free-living infective larvae lose a surface coat during penetration of the skin were further investigated by transmission electron microscopy of the cuticle of S. ratti infective larvae in the free-living stage, after penetration of mouse skin, and after migration to the lungs. These studies demonstrated the presence of a faint electron-dense surface coat external to the epicuticle on free-living worms which was absent from larvae recovered from the skin and lungs. When free-living infective larvae were incubated in 10% CO2 at 37 C and then examined with phase-contrast microscopy, worms were observed in the process of losing this coat. These observations confirm the hypothesis that S. ratti infective larvae lose a surface coat during penetration of the skin.
以往使用放射性标记幼虫的研究表明，斯氏类圆线虫自由生活的感染性幼虫在穿透皮肤时会失去表面涂层。通过对自由生活阶段、穿透小鼠皮肤后以及迁移至肺部后的斯氏类圆线虫感染性幼虫角质层进行透射电子显微镜观察，对这一现象进行了进一步研究。这些研究表明，自由生活的蠕虫在表皮外存在一层微弱的电子致密表面涂层，而从皮肤和肺部回收的幼虫中则没有。当将自由生活的感染性幼虫在37℃、10%二氧化碳环境中孵育，然后用相差显微镜检查时，观察到蠕虫正在失去这层涂层。这些观察结果证实了斯氏类圆线虫感染性幼虫在穿透皮肤时会失去表面涂层这一假说。
