Shi Xiaojie, Zhao Feiyang, Sun Huzhi, Yu Xiaoyan, Zhang Can, Liu Wenhua, Pan Qiang, Ren Huiying
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
Qingdao Phagepharm Bio-tech Co, Ltd, Qingdao, Shandong, China.
Curr Microbiol. 2020 Sep;77(9):2465-2474. doi: 10.1007/s00284-020-02011-5. Epub 2020 May 4.
A lytic Pseudomonas aeruginosa phage vB_PaeP_LP14 belonging to the family Podoviridae was isolated from infected mink. The microbiological characterization revealed that LP14 was stable at 40 to 50 °C and stable over a broad range of pH (5 to 12). The latent period was 5 min, and the burst size was 785 pfu/infected cell. The whole-genome sequencing showed that LP14 was a dsDNA virus and has a genome of 73,080 bp. The genome contained 93 predicted open reading frames (ORFs), 17 of which have known functions including DNA replication and modification, transcriptional regulation, structural and packaging proteins, and host cell lysis. No tRNA genes were identified. BLASTn analysis revealed that phage LP14 had a high-sequence identity (96%) with P. aeruginosa phage YH6. Both morphological characterization and genome annotation indicate that phage LP14 is a memberof the family Podoviridae genus Litunavirus. The study of phage LP14 will provide basic information for further research on treatment of P. aeruginosa infections.
从感染的水貂中分离出一种属于短尾病毒科的溶菌性铜绿假单胞菌噬菌体vB_PaeP_LP14。微生物学特性表明，LP14在40至50°C下稳定，在广泛的pH范围（5至12）内稳定。潜伏期为5分钟，裂解量为785个噬菌斑形成单位/感染细胞。全基因组测序表明，LP14是一种双链DNA病毒，基因组大小为73,080 bp。该基因组包含93个预测的开放阅读框（ORF），其中17个具有已知功能，包括DNA复制和修饰、转录调控、结构和包装蛋白以及宿主细胞裂解。未鉴定到tRNA基因。BLASTn分析表明，噬菌体LP14与铜绿假单胞菌噬菌体YH6具有高度序列同一性（96%）。形态学特征和基因组注释均表明噬菌体LP14是Litunavirus属短尾病毒科的成员。对噬菌体LP14的研究将为进一步研究铜绿假单胞菌感染的治疗提供基础信息。
