• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于单细胞测序鉴定 B 细胞标记基因，建立预后模型并鉴定骨肉瘤中的免疫浸润。

Identification of B cell marker genes based on single-cell sequencing to establish a prognostic model and identify immune infiltration in osteosarcoma.

机构信息

Department of Bone and Soft Tissue Tumors, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Department of Maxillofacial and Otorhinolaryngological Oncology, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer, Prevention and Therapy, Tianjin Cancer Institute, National Clinical Research Center of Cancer, Tianjin, China.

出版信息

Front Immunol. 2022 Dec 7;13:1026701. doi: 10.3389/fimmu.2022.1026701. eCollection 2022.

DOI:10.3389/fimmu.2022.1026701
PMID:36569871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9774034/
Abstract

BACKGROUND

Tumor-infiltrating B cells play a crucial role in the promotion or inhibition of tumor development. However, the role of B cells in osteosarcoma remains largely unknown. The aim of this study was to investigate the effect of B cells on the prognosis and immunity infiltration of osteosarcoma.

METHODS

Marker genes of B cells were identified based on the single-cell sequencing results of osteosarcoma in the GEO database. The prognostic model was established by the TCGA database and verified by the GEO data. The divergence in immune infiltration between the low-risk and high-risk groups was then compared according to the established prognostic model. Finally, the differential genes in the low-risk and high-risk groups were enriched and analyzed.

RESULTS

A total of 261 B cell marker genes was obtained by single-cell sequencing and a prognostic model of 4 B cell marker genes was established based on TCGA data. The model was found to have a good prediction performance in the TCGA and GEO data. A remarkable difference in immune infiltration between the low-risk and high-risk groups was also observed. The obtained results were verified by enrichment analysis.

CONCLUSION

In summary, a prognostic model with good predictive performance was established that revealed the indispensable role of B cells in the development of osteosarcoma. This model also provides a predictive index and a novel therapeutic target for immunotherapy for clinical patients.

摘要

背景

肿瘤浸润 B 细胞在促进或抑制肿瘤发展方面发挥着关键作用。然而，B 细胞在骨肉瘤中的作用在很大程度上仍不清楚。本研究旨在探讨 B 细胞对骨肉瘤预后和免疫浸润的影响。

方法

基于 GEO 数据库中骨肉瘤的单细胞测序结果，确定 B 细胞的标记基因。利用 TCGA 数据库建立预后模型，并通过 GEO 数据进行验证。然后根据建立的预后模型比较低风险组和高风险组之间免疫浸润的差异。最后，对低风险组和高风险组之间的差异基因进行富集分析。

结果

通过单细胞测序获得了 261 个 B 细胞标记基因，并基于 TCGA 数据建立了一个由 4 个 B 细胞标记基因组成的预后模型。该模型在 TCGA 和 GEO 数据中均具有良好的预测性能。低风险组和高风险组之间的免疫浸润也存在显著差异。通过富集分析验证了这些结果。

结论

总之，建立了一个具有良好预测性能的预后模型，揭示了 B 细胞在骨肉瘤发生发展中的不可或缺的作用。该模型还为临床患者的免疫治疗提供了一个预测指标和一个新的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/77744b76f994/fimmu-13-1026701-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/ceb9f7a7e767/fimmu-13-1026701-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/e767484a56d0/fimmu-13-1026701-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/0ae5efbb74b9/fimmu-13-1026701-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/56dd27a0b036/fimmu-13-1026701-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/affb88566e98/fimmu-13-1026701-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/e0cd0d47bbc3/fimmu-13-1026701-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/5758024cbe0d/fimmu-13-1026701-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/ece53610cac5/fimmu-13-1026701-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/3a851b46b4f8/fimmu-13-1026701-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/b2beb92102b2/fimmu-13-1026701-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/77744b76f994/fimmu-13-1026701-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/ceb9f7a7e767/fimmu-13-1026701-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/e767484a56d0/fimmu-13-1026701-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/0ae5efbb74b9/fimmu-13-1026701-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/56dd27a0b036/fimmu-13-1026701-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/affb88566e98/fimmu-13-1026701-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/e0cd0d47bbc3/fimmu-13-1026701-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/5758024cbe0d/fimmu-13-1026701-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/ece53610cac5/fimmu-13-1026701-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/3a851b46b4f8/fimmu-13-1026701-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/b2beb92102b2/fimmu-13-1026701-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace3/9774034/77744b76f994/fimmu-13-1026701-g011.jpg

相似文献

1
Identification of B cell marker genes based on single-cell sequencing to establish a prognostic model and identify immune infiltration in osteosarcoma.基于单细胞测序鉴定 B 细胞标记基因，建立预后模型并鉴定骨肉瘤中的免疫浸润。
Front Immunol. 2022 Dec 7;13:1026701. doi: 10.3389/fimmu.2022.1026701. eCollection 2022.
2
A novel molecular signature for predicting prognosis and immunotherapy response in osteosarcoma based on tumor-infiltrating cell marker genes.基于肿瘤浸润细胞标志物基因的骨肉瘤预后和免疫治疗反应的新型分子特征。
Front Immunol. 2023 Apr 6;14:1150588. doi: 10.3389/fimmu.2023.1150588. eCollection 2023.
3
Construction of an oxidative phosphorylation-related gene signature for predicting prognosis and identifying immune infiltration in osteosarcoma.构建与氧化磷酸化相关的基因特征，用于预测骨肉瘤的预后和鉴定免疫浸润。
Aging (Albany NY). 2024 Mar 20;16(6):5311-5335. doi: 10.18632/aging.205650.
4
Identification and characterization of aging/senescence-induced genes in osteosarcoma and predicting clinical prognosis.鉴定和特征分析骨肉瘤中与衰老/衰老相关的基因，并预测临床预后。
Front Immunol. 2022 Oct 5;13:997765. doi: 10.3389/fimmu.2022.997765. eCollection 2022.
5
ADAM19 and TUBB1 Correlate with Tumor Infiltrating Immune Cells and Predicts Prognosis in Osteosarcoma.ADAM19 和 TUBB1 与肿瘤浸润免疫细胞相关，并预测骨肉瘤的预后。
Comb Chem High Throughput Screen. 2023;26(1):135-148. doi: 10.2174/1386207325666220406112305.
6
Construction of a prognostic risk score model based on the ARHGAP family to predict the survival of osteosarcoma.基于 ARHGAP 家族构建预后风险评分模型预测骨肉瘤患者的生存情况。
BMC Cancer. 2023 Dec 1;23(1):1179. doi: 10.1186/s12885-023-11673-w.
7
Development of an immune-related prognostic index associated with osteosarcoma.免疫相关预后指数与骨肉瘤相关的发展。
Bioengineered. 2021 Dec;12(1):172-182. doi: 10.1080/21655979.2020.1864096.
8
The hexosamine biosynthesis pathway-related gene signature correlates with immune infiltration and predicts prognosis of patients with osteosarcoma.己糖胺生物合成途径相关基因特征与免疫浸润相关，并可预测骨肉瘤患者的预后。
Front Immunol. 2022 Oct 6;13:1028263. doi: 10.3389/fimmu.2022.1028263. eCollection 2022.
9
Osteosarcoma-Associated Immune Genes as Potential Immunotherapy and Prognosis Biomarkers.骨肉瘤相关免疫基因作为潜在的免疫治疗和预后生物标志物。
Biochem Genet. 2024 Apr;62(2):798-813. doi: 10.1007/s10528-023-10444-3. Epub 2023 Jul 14.
10
Construction and validation of an oxidative-stress-related risk model for predicting the prognosis of osteosarcoma.构建并验证一个与氧化应激相关的风险模型，用于预测骨肉瘤的预后。
Aging (Albany NY). 2023 Jun 2;15(11):4820-4843. doi: 10.18632/aging.204764.

引用本文的文献

1
Tumor microenvironment in osteosarcoma: From cellular mechanism to clinical therapy.骨肉瘤中的肿瘤微环境：从细胞机制到临床治疗
Genes Dis. 2025 Feb 20;12(5):101569. doi: 10.1016/j.gendis.2025.101569. eCollection 2025 Sep.
2
Osteoimmunology in bone malignancies: a symphony with evil.骨恶性肿瘤中的骨免疫学：与邪恶的交响曲。
J Natl Cancer Cent. 2024 Sep 14;4(4):354-368. doi: 10.1016/j.jncc.2024.09.001. eCollection 2024 Dec.
3
Deciphering the prognostic and therapeutic significance of BAG1 and BAG2 for predicting distinct survival outcome and effects on liposarcoma.

本文引用的文献

1
Single-cell RNA sequencing reveals differential expression of EGFL7 and VEGF in giant-cell tumor of bone and osteosarcoma.单细胞 RNA 测序揭示骨巨细胞瘤和骨肉瘤中 EGFL7 和 VEGF 的差异表达。
Exp Biol Med (Maywood). 2022 Jul;247(14):1214-1227. doi: 10.1177/15353702221088238. Epub 2022 Jun 13.
2
Identification of a seven-gene prognostic signature using the gene expression profile of osteosarcoma.利用骨肉瘤基因表达谱鉴定七基因预后特征
Ann Transl Med. 2022 Jan;10(2):53. doi: 10.21037/atm-21-6276.
3
Intratumoral plasma cells predict outcomes to PD-L1 blockade in non-small cell lung cancer.
解析 BAG1 和 BAG2 对预测不同生存结局和脂肪肉瘤治疗效果的预后及治疗意义。
Sci Rep. 2024 Oct 4;14(1):23084. doi: 10.1038/s41598-024-67659-6.
4
Exosomal mRNA Cargo are biomarkers of tumor and immune cell populations in pediatric osteosarcoma.外泌体mRNA货物是小儿骨肉瘤中肿瘤和免疫细胞群体的生物标志物。
Transl Oncol. 2024 Aug;46:102008. doi: 10.1016/j.tranon.2024.102008. Epub 2024 Jun 8.
5
Decoding the Impact of Tumor Microenvironment in Osteosarcoma Progression and Metastasis.解析肿瘤微环境在骨肉瘤进展和转移中的影响
Cancers (Basel). 2023 Oct 23;15(20):5108. doi: 10.3390/cancers15205108.
肿瘤内浆细胞可预测非小细胞肺癌患者对程序性死亡受体-1配体（PD-L1）阻断治疗的反应。
Cancer Cell. 2022 Mar 14;40(3):289-300.e4. doi: 10.1016/j.ccell.2022.02.002. Epub 2022 Feb 24.
4
Construction and validation of a novel gene signature for predicting the prognosis of osteosarcoma.构建并验证用于预测骨肉瘤预后的新型基因标志物。
Sci Rep. 2022 Jan 24;12(1):1279. doi: 10.1038/s41598-022-05341-5.
5
Expression of immune-related genes as prognostic biomarkers for the assessment of osteosarcoma clinical outcomes.免疫相关基因的表达作为评估骨肉瘤临床结局的预后生物标志物。
Sci Rep. 2021 Dec 16;11(1):24123. doi: 10.1038/s41598-021-03677-y.
6
Neoantigen-driven B cell and CD4 T follicular helper cell collaboration promotes anti-tumor CD8 T cell responses.新抗原驱动的 B 细胞和 CD4+T 滤泡辅助细胞协同作用促进抗肿瘤 CD8+T 细胞反应。
Cell. 2021 Dec 9;184(25):6101-6118.e13. doi: 10.1016/j.cell.2021.11.007. Epub 2021 Nov 30.
7
B cells and cancer.B细胞与癌症。
Cancer Cell. 2021 Oct 11;39(10):1293-1296. doi: 10.1016/j.ccell.2021.09.007. Epub 2021 Sep 30.
8
Identification of a Novel Prognostic Gene Signature From the Immune Cell Infiltration Landscape of Osteosarcoma.从骨肉瘤免疫细胞浸润图谱中鉴定一种新的预后基因特征
Front Cell Dev Biol. 2021 Sep 6;9:718624. doi: 10.3389/fcell.2021.718624. eCollection 2021.
9
Ferroptosis-related gene signature associates with immunity and predicts prognosis accurately in patients with osteosarcoma.铁死亡相关基因特征与免疫相关，可准确预测骨肉瘤患者的预后。
Cancer Sci. 2021 Nov;112(11):4785-4798. doi: 10.1111/cas.15131. Epub 2021 Sep 21.
10
Role of B Cells in Responses to Checkpoint Blockade Immunotherapy and Overall Survival of Cancer Patients.B 细胞在癌症患者对免疫检查点阻断治疗的反应和总体生存中的作用。
Clin Cancer Res. 2021 Nov 15;27(22):6075-6082. doi: 10.1158/1078-0432.CCR-21-0697. Epub 2021 Jul 6.