Zhou Ping, Pan Yingbo, Pan Wei, Lu Sumei, Yin Jiaqi, Li Na, Tang Bo
College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China.
Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Medicine and Health Key Laboratory of Laboratory Medicine, Jinan 250014, Shandong, P. R. China.
Anal Chem. 2023 Jan 17;95(2):1280-1286. doi: 10.1021/acs.analchem.2c04121. Epub 2022 Dec 27.
The detection of circulating tumor microRNAs (miRNAs) holds great promise for the noninvasive and early-stage diagnosis of cancer. However, the low abundance of lung cancer-related miRNAs and the false-positive results of single miRNA detection limited the development of strip-based point-of-care testing methods in clinic. We developed a duplex-specific nuclease (DSN)-mediated and dual-AND logic gate-based triple-line lateral flow strip detection system for the rapid and simultaneous detection of four miRNAs of lung cancer in a single strip test. This system combines DSN-mediated signal amplification with AND logic gate-based simple signal output. Meanwhile, the limit of detection of this platform was calculated to be 26.51 fM. Furthermore, this assay was used to detect lung cancer-related miRNAs from serum in a homogeneous and separation-free format, which could discriminate lung cancer patients from healthy individuals with an accuracy of 100%. Our approach provides a simple and easy-to-handle method for the diagnosis of lung cancer in clinic.
循环肿瘤微小RNA（miRNA）的检测在癌症的非侵入性和早期诊断方面具有巨大潜力。然而，肺癌相关miRNA的低丰度以及单一miRNA检测的假阳性结果限制了基于试纸条的即时检测方法在临床中的发展。我们开发了一种基于双链特异性核酸酶（DSN）介导和双与逻辑门的三线侧向流动试纸条检测系统，用于在单次试纸条检测中快速同时检测四种肺癌miRNA。该系统将DSN介导的信号放大与基于与逻辑门的简单信号输出相结合。同时，该平台的检测限经计算为26.51 fM。此外，该检测方法用于以均相且无需分离的形式检测血清中肺癌相关的miRNA，能够以100%的准确率区分肺癌患者和健康个体。我们的方法为临床肺癌诊断提供了一种简单且易于操作的方法。
