Zhao Xiaoping, Yang Zhiwei, Niu Ruoxin, Tang Ye, Wang Heng, Gao Rui, Zhao Yizhen, Jing Xunan, Wang Daquan, Lin Peng, Guan Hao, Meng Lingjie
School of Chemistry, Xi'an Key Laboratory of Sustainable Energy Material Chemistry, Xi'an Jiaotong University, Xi'an710049, P.R. China.
MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an710049, P.R. China.
Anal Chem. 2023 Jan 17;95(2):1731-1738. doi: 10.1021/acs.analchem.2c05043. Epub 2022 Dec 28.
The COVID-19 pandemic has spread to every corner of the world and seriously affected our health and daily activities in the past three years; thereby, it is still urgent to develop various simple, quick, and accurate methods for early detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. Nanozymes, a kind of nanomaterial with intrinsic enzyme-mimicking activity, have emerged as a suitable alternative for both therapy and diagnosis of SARS-CoV-2. Here, ultrasensitive and ultrafast MIL-101(CuFe)-CD147 biosensors are established for the detection of SARS-CoV-2 by a simple colorimetric method. A MIL-101(CuFe) metal-organic framework has excellent peroxidase-like activity due to the synergistic effect of Fe and Cu atoms. In addition, the MIL-101(CuFe)-CD147 biosensor shows great potential to detect the various variants of SARS-CoV-2 due to the universal receptor of CD147. The enzyme-based biosensor for the detection of SARS-CoV-2 achieves a very low limit of detection (about 3 PFU/mL) within 30 min. Therefore, the present method provides a new generation of an alternative approach for highly sensitive and visual diagnosis of COVID-19.
在过去三年里，新冠疫情已蔓延至世界各个角落，并严重影响了我们的健康和日常活动；因此，开发各种简单、快速且准确的方法用于早期检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）传播仍然十分紧迫。纳米酶是一种具有内在类酶活性的纳米材料，已成为治疗和诊断SARS-CoV-2的合适替代物。在此，通过一种简单的比色法建立了用于检测SARS-CoV-2的超灵敏且超快的MIL-101（CuFe）-CD147生物传感器。由于铁和铜原子的协同作用，MIL-101（CuFe）金属有机框架具有优异的过氧化物酶样活性。此外，由于CD147的通用受体，MIL-101（CuFe）-CD147生物传感器在检测SARS-CoV-2的各种变体方面显示出巨大潜力。用于检测SARS-CoV-2的基于酶的生物传感器在30分钟内实现了极低的检测限（约3 PFU/mL）。因此本方法为新冠病毒的高灵敏度和可视化诊断提供了新一代的替代方法。
