Fu Hao, Bai Zhenyu, Li Ping, Feng Xiaoyang, Hu Xueping, Song Xingliang, Chen Lingxin
School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.
School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.
Food Chem. 2023 May 30;409:135292. doi: 10.1016/j.foodchem.2022.135292. Epub 2022 Dec 23.
Ovalbumin (OVA), a class of glycoproteins, is the main allergen in hen egg white that often causes allergies in humans, especially in babies. Therefore, it is pivotal to be able to detect and separate OVA. This work presents an ingenious sandwich-structured magnetic molecular imprinted electrochemical sensor for OVA detection by utilizing boronate affinity and signal amplification strategy. With anti-OVA antibody-modified gold nanoparticles (AuNPs) as amplifiers, the imprinted cavities in the probe could capture protein to form a sandwich structure. Due to its specific recognition of antibody and molecular imprinted polymers and the signal amplification of AuNPs, the sensor had good selectivity and sensitivity toward OVA and a low detection limit of 3.0 fg/mL. The sensor also had excellent stability and could satisfactorily detect OVA in real red wine samples.
卵清蛋白（OVA）是一类糖蛋白，是鸡蛋清中的主要过敏原，常引起人类过敏，尤其是婴儿。因此，能够检测和分离OVA至关重要。这项工作提出了一种巧妙的三明治结构磁性分子印迹电化学传感器，用于通过硼酸盐亲和作用和信号放大策略检测OVA。以抗OVA抗体修饰的金纳米粒子（AuNPs）作为放大器，探针中的印迹腔可以捕获蛋白质形成三明治结构。由于其对抗体和分子印迹聚合物的特异性识别以及AuNPs的信号放大作用，该传感器对OVA具有良好的选择性和灵敏度，检测限低至3.0 fg/mL。该传感器还具有出色的稳定性，能够令人满意地检测实际红酒样品中的OVA。
