Rhein-Knudsen Nanna, Reyes-Weiss Diego, Horn Svein Jarle
Faculty of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences (NMBU), P.O. Box 5003, 1432 Ås, Norway.
Faculty of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences (NMBU), P.O. Box 5003, 1432 Ås, Norway.
Int J Biol Macromol. 2023 Feb 28;229:199-209. doi: 10.1016/j.ijbiomac.2022.12.261. Epub 2022 Dec 28.
Fucoidans are fucose rich sulfated polysaccharides that are found in the cell wall of brown seaweeds and have been shown to have several beneficial bioactivities. In the present study, we report a new enzymatic extraction technique for the production of pure and intact fucoidans from the two brown seaweeds Saccharina latissima and Alaria esculenta. This new extraction protocol uses the commercial cellulase blend Cellic® CTec2 in combination with endo- and exo-acting thermophilic alginate lyases. The fucoidans obtained by this extraction technique are compared to traditionally extracted fucoidans in terms of chemical compositions and molecular weights and are shown to contain significantly higher amounts of fucose and sulfate, the main components of fucoidans, while cellulose, laminarin, and alginate contamination is low. Thus, by using this combination of enzymes, the extracted fucoidans do not undergo depolymerization during extraction and additional purification steps are not needed. The high purity fucoidans isolated by this new enzymatic extraction technique can be used to provide insight into the different fucoidan structures and biological activities.
岩藻依聚糖是富含岩藻糖的硫酸化多糖，存在于褐藻的细胞壁中，已被证明具有多种有益的生物活性。在本研究中，我们报告了一种新的酶促提取技术，用于从两种褐藻——海带（Saccharina latissima）和掌状海带（Alaria esculenta）中生产纯净且完整的岩藻依聚糖。这种新的提取方案使用商业纤维素酶混合物Cellic® CTec2与内切和外切嗜热海藻酸裂合酶相结合。通过这种提取技术获得的岩藻依聚糖在化学组成和分子量方面与传统提取的岩藻依聚糖进行了比较，结果表明其岩藻糖和硫酸盐（岩藻依聚糖的主要成分）含量显著更高，而纤维素、海带多糖和海藻酸盐的污染较低。因此，通过使用这种酶组合，提取的岩藻依聚糖在提取过程中不会发生解聚，也不需要额外的纯化步骤。通过这种新的酶促提取技术分离出的高纯度岩藻依聚糖可用于深入了解不同的岩藻依聚糖结构和生物活性。
