Massilia phyllostachyos sp. nov., Isolated from the Roots of Moso Bamboo in China.

A Gram-negative, strictly aerobic, motile, and rod-shaped bacterial strain G4R7 was isolated from the roots of moso bamboo (Phyllostachys edulis) in Zhejiang, Hangzhou Province, China. After comparing 16S rRNA gene sequences, strain G4R7 exhibited the highest similarities with Massilia neuiana PTW21 (98.3%), followed by M. agri K-3-1 (98.3%), M. consociate CCUG 58010 (97.7%), M. niastensis 5516S-1 (97.7%) and M. yuzhufengensis ZD1-4 (97.6%). The phylogenetic analysis revealed that strain G4R7 belonged to the genus Massilia. The draft genome of strain G4R7 was 5.81 Mb, and the G+C content was 64.4%. The average nucleotide identity values between G4R7 and another related member of the genus Massilia ranged from 76.6 to 87.2%, and the digital DNA-DNA hybridization ranged from 20.7 to 27.9%. Strain G4R7 grew at 15-37 °C (optimum 25-30 °C) and pH 6.0-9.0 (optimum pH 7.0) in the presence of 0-3% (w/v) NaCl (optimum 0%). The respiratory quinone was Q-8, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and aminophospholipid. The major cellular fatty acids were C 3OH, C, C 2OH, and C, summed feature 3 (C ω6c and/or C ω7c). As per the data from chemotaxonomic, phylogenetic, and phenotypic evidence, strain G4R7 represents a new species of genus Massilia, for which the name Massilia phyllostachyos sp. nov. is proposed. The type strain is G4R7 (=ACCC 61911=GDMCC 1.2961=JCM 35225).