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sp. nov.,分离自土壤。

sp. nov., isolated from soil.

机构信息

Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2022 Feb;72(2). doi: 10.1099/ijsem.0.005227.

Abstract

A Gram-stain-negative, catalase- and oxidase-positive and aerobic bacterium, designated strain R798, was isolated from soil in South Korea. Cells were motile rods by means of a single polar flagellum. Growth of strain R798 was observed at 15-35 °C (optimum, 25-30 °C), pH 5.0-8.0 (optimum, 6.0) and 0-1.5 % NaCl (optimum, 0.3 %). Strain R798 contained ubiquinone-8 as the sole isoprenoid quinone, summed feature 3 (C 7 and/or C 6) and C as the major fatty acids and phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The DNA G+C content of strain R798 calculated from the whole genome sequence was 63.3 mol%. Phylogenetic analyses based on the 16S rRNA gene and whole genome sequences revealed that strain R798 formed a distinct phyletic lineage within the genus . Strain R798 was most closely related to B528-3 with a 98.0 % 16S rRNA gene sequence similarity. Average nucleotide identity and digital DNA-DNA hybridization values between strain R798 and the type strain of were 79.2 and 22.7 %, respectively. Based on the phenotypic, chemotaxonomic and molecular analyses, strain R798 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R798 (=KACC 22114=JCM 34601).

摘要

一株革兰氏阴性、接触酶和氧化酶阳性、需氧的细菌,被命名为 R798 菌株,从韩国的土壤中分离得到。细胞通过单一极生鞭毛运动。R798 菌株的生长在 15-35°C(最佳,25-30°C)、pH 值 5.0-8.0(最佳,6.0)和 0-1.5%NaCl(最佳,0.3%)下观察到。R798 菌株含有醌-8 作为唯一的异戊二烯醌,总和特征 3(C7 和/或 C6)和 C 作为主要脂肪酸和磷脂酰甘油和磷脂酰乙醇胺作为主要极性脂。根据全基因组序列计算,R798 菌株的 DNA G+C 含量为 63.3mol%。基于 16S rRNA 基因和全基因组序列的系统发育分析表明,R798 菌株在 属内形成了一个独特的系统发育谱系。R798 菌株与 B528-3 最为密切相关,16S rRNA 基因序列相似度为 98.0%。R798 菌株与模式菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 79.2%和 22.7%。基于表型、化学分类和分子分析,R798 菌株代表了 属的一个新种,建议将其命名为 sp. nov.。模式菌株为 R798(=KACC 22114=JCM 34601)。

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