Biomaterials and Biotechnology Laboratory, Science and Technology Research and Application Center (BITAM), Necmettin Erbakan University, 42140 Konya, Turkey.
Biomaterials and Biotechnology Laboratory, Science and Technology Research and Application Center (BITAM), Necmettin Erbakan University, 42140 Konya, Turkey; Department of Chemistry Education, A.K. Education Faculty, Necmettin Erbakan University, 42090 Konya, Turkey.
J Pharm Biomed Anal. 2023 Feb 20;225:115206. doi: 10.1016/j.jpba.2022.115206. Epub 2022 Dec 20.
Early diagnosis saves lives in many diseases. In this sense, monitoring of biomarkers is crucial for the diagnosis of diseases. Lateral flow assays (LFAs) have attracted great attention among paper-based point-of-care testing (POCT) due to their low cost, user-friendliness, and time-saving advantages. Developments in the field of health have led to an increase of interest in these rapid tests. LFAs are used in the diagnosis and monitoring of many diseases, thanks to biomarkers that can be observed in body fluids. This review covers the recent advances dealing with the design and strategies for the development of LFA for the detection of biomarkers used in clinical applications in the last 5 years. We focus on various strategies such as choosing the nanoparticle type, single or multiple test approaches, and equipment for signal transducing for the detection of the most common biomarkers in different diseases such as cancer, cardiovascular, infectious, and others including Parkinson's and Alzheimer's diseases. We expect that this study will contribute to the different approaches in LFA and pave the way for other clinical applications.
早期诊断可以挽救许多疾病患者的生命。从这个意义上说，生物标志物的监测对于疾病的诊断至关重要。由于具有成本低、使用方便和节省时间等优点，基于纸条的即时检测（POCT）中的侧向流动分析（LFA）受到了极大关注。由于健康领域的发展，人们对这些快速检测的兴趣日益增加。LFA 可用于许多疾病的诊断和监测，这要归功于可以在体液中观察到的生物标志物。本文综述了过去 5 年中用于临床应用的生物标志物检测的 LFA 设计和开发的最新进展。我们专注于各种策略，例如选择纳米颗粒类型、单一或多种测试方法以及用于信号转换的设备，以检测癌症、心血管疾病、传染病等不同疾病中最常见的生物标志物，以及帕金森病和阿尔茨海默病等其他疾病。我们希望这项研究将有助于 LFA 的不同方法，并为其他临床应用铺平道路。
