State Key Laboratory of Food Science and Technology, Jiangnan University, No. 1800, Lihu Road, Wuxi, Jiangsu, 214122, P. R. China.
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, No. 1800, Lihu Road, Wuxi, Jiangsu, 214122, P. R. China.
Small Methods. 2022 Jan;6(1):e2101143. doi: 10.1002/smtd.202101143. Epub 2021 Dec 2.
The development of the lateral flow assay (LFA) has received much attention in both academia and industry because of their broad applications to food safety, environmental monitoring, clinical diagnosis, and so forth. The user friendliness, low cost, and easy operation are the most attractive advantages of the LFA. In recent years, quantitative detection has become another focus of LFA development. Here, the most recent studies of quantitative LFAs are reviewed. First, the principles and corresponding formats of quantitative LFAs are introduced. In the biomaterial and nanomaterial sections, the detection, capture, and signal amplification biomolecules and the optical, fluorescent, luminescent, and magnetic labels used in LFAs are described. The invention of dedicated strip readers has drawn further interest in exploiting the better performance of LFAs. Therefore, next, the development of dedicated reader devices is described and the usefulness and specifications of these devices for LFAs are discussed. Finally, the applications of LFAs in the detection of metal ions, biotoxins, pathogenic microorganisms, veterinary drugs, and pesticides in the fields of food safety and environmental health and the detection of nucleic acids, biomarkers, and viruses in clinical analyses are summarized.
侧向流检测（LFA）的发展在学术界和工业界都受到了广泛关注，因为它在食品安全、环境监测、临床诊断等方面有广泛的应用。LFA 的最大优势在于其用户友好性、低成本和易于操作。近年来，定量检测已成为 LFA 发展的另一个重点。本文综述了最近关于定量 LFA 的研究。首先，介绍了定量 LFA 的原理和相应的格式。在生物材料和纳米材料部分，描述了 LFA 中用于检测、捕获和信号放大的生物分子，以及光学、荧光、发光和磁性标记物。专用条带读取器的发明进一步引起了人们对开发更好性能 LFA 的兴趣。因此，接下来描述了专用读取器设备的开发，并讨论了这些设备在 LFA 中的实用性和规格。最后，总结了 LFA 在食品安全和环境卫生领域检测金属离子、生物毒素、致病微生物、兽药和农药，以及在临床分析中检测核酸、生物标志物和病毒方面的应用。
