The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, PR China.
Department of Chemistry and Biochemistry, Biomedical Engineering, Border Biomedical Research Center, Environmental Science and Engineering, University of Texas at El Paso, 500 West University Avenue, El Paso, TX, 79968, United States.
Talanta. 2019 Aug 15;201:126-133. doi: 10.1016/j.talanta.2019.03.105. Epub 2019 Apr 3.
Upconversion nanoparticle-based lateral flow assays (UCNP-LFAs) have attracted significant attention in point-of-care testing (POCT) applications, due to the long-term photostability and enhanced signal-to-background noise ratio. The existing UCNP-LFAs generally require peripheral equipment for exciting fluorescent signals and reading out fluorescence results, which are generally bulky and expensive. Herein, we developed a miniaturized and portable UCNP-LFA platform, which is composed of a LFA detection system, an UCNP-LFA reader and a smartphone-assisted UCNP-LFA analyzer. The LFA detection system is based on three types of UCNPs for multiplexed detection. The reader has a dimension of 24.0 cm × 9.4 cm × 5.4 cm (L × W × H) and weight of 0.9 kg. The analyzer based on the custom-designed software of a smartphone (termed as UCNP-LFA analyzer) can get the quantitative analysis results in a real-time manner. We demonstrated the universality of this platform by highly sensitive and quantitative detections of several kinds of targets, including small molecule (ochratoxin A, OTA), heavy metal ion (Hg), bacteria (salmonella, SE), nucleic acid (hepatitis B virus, HBV) and protein (growth stimulation expressed gene 2, ST-2). Our developed UCNP-LFA platform holds great promise for applications in disease diagnostics, environmental pollution monitoring and food safety at the point of care.
上转换纳米粒子侧向流分析（UCNP-LFA）因其长期的光稳定性和增强的信背比，在即时检测（POCT）应用中引起了极大的关注。现有的 UCNP-LFA 通常需要外围设备来激发荧光信号并读取荧光结果，这些设备通常体积庞大且昂贵。在此，我们开发了一种微型化和便携式的 UCNP-LFA 平台，它由 LFA 检测系统、UCNP-LFA 读取器和智能手机辅助的 UCNP-LFA 分析仪组成。LFA 检测系统基于三种类型的上转换纳米粒子进行多重检测。读取器的尺寸为 24.0 cm×9.4 cm×5.4 cm（L×W×H），重量为 0.9 kg。基于智能手机定制软件的分析仪（称为 UCNP-LFA 分析仪）可以实时获得定量分析结果。我们通过对几种目标物的高灵敏度和定量检测，证明了该平台的通用性，这些目标物包括小分子（赭曲霉毒素 A，OTA）、重金属离子（汞）、细菌（沙门氏菌，SE）、核酸（乙型肝炎病毒，HBV）和蛋白质（生长刺激表达基因 2，ST-2）。我们开发的 UCNP-LFA 平台有望在疾病诊断、环境污染监测和即时医疗点的食品安全等领域得到应用。
