School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam 690525 Kerala, India.
Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru 560029, Karnataka, India.
Bioresour Technol. 2023 Feb;370:128555. doi: 10.1016/j.biortech.2022.128555. Epub 2022 Dec 28.
The gradually increasing need for fossil fuels demands renewable biofuel substitutes. This has fascinated an increasing investigation to design innovative energy fuels that have comparable Physico-chemical and combustion characteristics with fossil-derived fuels. The efficient microbes for bioenergy synthesis desire the proficiency to consume a large quantity of carbon substrate, transfer various carbohydrates through efficient metabolic pathways, capability to withstand inhibitory components and other degradation compounds, and improve metabolic fluxes to synthesize target compounds. Metabolically engineered microbes could be an efficient methodology for synthesizing biofuel from cellulosic biomass by cautiously manipulating enzymes and metabolic pathways. This review offers a comprehensive perspective on the trends and advances in metabolic and genetic engineering technologies for advanced biofuel synthesis by applying various heterologous hosts. Probable technologies include enzyme engineering, heterologous expression of multiple genes, CRISPR-Cas technologies for genome editing, and cell surface display.
对化石燃料的需求逐渐增加，这就要求可再生生物燃料替代品的出现。这引起了人们越来越多的关注，旨在设计具有与化石衍生燃料相当的物理化学和燃烧特性的创新能源燃料。生物能源合成的高效微生物需要能够消耗大量的碳底物，通过有效的代谢途径转化各种碳水化合物，能够承受抑制性成分和其他降解化合物，并提高代谢通量以合成目标化合物。代谢工程微生物可能是一种通过谨慎操纵酶和代谢途径来从纤维素生物质合成生物燃料的有效方法。本综述通过应用各种异源宿主，全面介绍了代谢和遗传工程技术在先进生物燃料合成方面的趋势和进展。可能的技术包括酶工程、多个基因的异源表达、基因组编辑的 CRISPR-Cas 技术和细胞表面展示。
