School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, Jiangsu, 223003, China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, China.
Chemosphere. 2022 Feb;288(Pt 2):132528. doi: 10.1016/j.chemosphere.2021.132528. Epub 2021 Oct 9.
Global warming issues, rapid fossil fuel diminution, and increasing worldwide energy demands have diverted accelerated attention in finding alternate sources of biofuels and energy to combat the energy crisis. Bioconversion of lignocellulosic biomass has emerged as a prodigious way to produce various renewable biofuels such as biodiesel, bioethanol, biogas, and biohydrogen. Ideal microbial hosts for biofuel synthesis should be capable of using high substrate quantity, tolerance to inhibiting substances and end-products, fast sugar transportation, and amplified metabolic fluxes to yielding enhanced fermentative bioproduct. Genetic manipulation and microbes' metabolic engineering are fascinating strategies for the economical production of next-generation biofuel from lignocellulosic feedstocks. Metabolic engineering is a rapidly developing approach to construct robust biofuel-producing microbial hosts and an important component for future bioeconomy. This approach has been widely adopted in the last decade for redirecting and revamping the biosynthetic pathways to obtain a high titer of target products. Biotechnologists and metabolic scientists have produced a wide variety of new products with industrial relevance through metabolic pathway engineering or optimizing native metabolic pathways. This review focuses on exploiting metabolically engineered microbes as promising cell factories for the enhanced production of advanced biofuels.
全球变暖问题、化石燃料的迅速减少以及全球能源需求的不断增长，促使人们加快寻找替代生物燃料和能源的方法，以应对能源危机。木质纤维素生物质的生物转化已成为生产各种可再生生物燃料（如生物柴油、生物乙醇、沼气和生物氢）的重要途径。理想的生物燃料合成微生物宿主应能够利用大量的底物、耐受抑制物质和终产物、快速的糖转运以及放大的代谢通量，以产生增强的发酵生物制品。遗传操作和微生物代谢工程是从木质纤维素原料经济生产下一代生物燃料的迷人策略。代谢工程是构建稳健的生物燃料生产微生物宿主的快速发展方法，也是未来生物经济的重要组成部分。在过去的十年中，该方法已被广泛用于重新定向和改造生物合成途径，以获得高浓度的目标产物。生物技术人员和代谢科学家已经通过代谢途径工程或优化天然代谢途径，生产出了具有工业相关性的各种新产品。本综述重点介绍了利用代谢工程化微生物作为有前途的细胞工厂，以提高先进生物燃料的产量。
